The Effect of Macrophage Addition to Interleukin 10 (IL-10) in Tuberculosis Granuloma Model In Vitro

Dini Puspodewi, Agung Dwi Wahyu W, Jusak Nugraha

2018

Abstract

Tuberculosis (TB) caused by the bacterium Mycobacterium tuberculosis has caused more deaths than other infectious diseases over the past 200 years. Granulomas are characteristic of mycobacterial infection. Doubling of Mycobacterium tuberculosis bacilli in any place will cause specific inflammation to form characteristic granulomas. Granulomas contains of different types of cells, but the main cellular component of the structure is macrophages. Macrophages are cells for the formation of granulomas and major cell types in most granulomas. One function of macrophages in granulomas is the production of anti-inflammatory cytokines IL-10. IL-10 can reduce inflammation and maintain homeostasis. This study aims to determine the effect of the addition of macrophages to levels of interleukin-10 (IL-10) in granuloma model in vitro. This type of research is true experimental with the object of research in the form of PBMC which comes from 1 healthy volunteer. Analysis using twoway ANNOVA with P <0.05, and tukey comparing for comparison. The results showed that without macrophage group with 1x105, 2x105, and 3x105 macrophage groups, the value of p = 0.3197 was greater than 0.05 (p> 0.05), so there was no significant difference. While the variation day in the treatment group obtained p = 0.2407 which showed no effect of macrophage addition to interleukin-10 (IL-10) level on granuloma model in vitro, with IL-10 concentration on the 1st day until the 5th day are low. There is no effect of adding macrophages to IL-10 levels in tuberculosis granuloma models in vitro

Paper Citation

in Harvard Style

Puspodewi D., W A. and Nugraha J. (2018). The Effect of Macrophage Addition to Interleukin 10 (IL-10) in Tuberculosis Granuloma Model In Vitro.In Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS, ISBN 978-989-758-348-3, pages 386-391. DOI: 10.5220/0007543503860391

in Bibtex Style

@conference{icps18,
author={Dini Puspodewi and Agung Dwi Wahyu W and Jusak Nugraha},
title={The Effect of Macrophage Addition to Interleukin 10 (IL-10) in Tuberculosis Granuloma Model In Vitro},
booktitle={Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,},
year={2018},
pages={386-391},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007543503860391},
isbn={978-989-758-348-3},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,
TI - The Effect of Macrophage Addition to Interleukin 10 (IL-10) in Tuberculosis Granuloma Model In Vitro
SN - 978-989-758-348-3
AU - Puspodewi D.
AU - W A.
AU - Nugraha J.
PY - 2018
SP - 386
EP - 391
DO - 10.5220/0007543503860391