Potential of Black Seed Oil (Nigella Sativa) as Immunomodulator in Mice (Mus Musculus) Infected with Salmonella Typhimurium

Eka Yunita Wulandari, Dwi Krihariyani, Suliati

2018

Abstract

Typhoid fever is a systemic disease mainly caused by Salmonella typhi that spreads systemically into blood circulation and leads to bacteria accumulation in the intestine. Black seed oil (Nigella sativa) contains a chemical substance called thymoquinone acting as immunomodulator that can enhance immune system to prevent bacteria colonization in the intestine. This study was to determine the potential of black seed oil as immunomodulator to mice infected with Salmonella typhimurium. This study is an experimental research using completely randomized design (CRD) with 4 treatments and 6 replications. Animals used were 2-month-old male mice, weighing 25 - 30 g totaling 24. Treatments in this study included K- (normal mice), K+ (mice infected with Salmonella typhimurium i.p), P1 (mice orally administered with black seed oil for 7 days and infected with Salmonella typhimurium i.p), and P2 (mice orally administered with black seed oil for 14 days and infected with Salmonella typhimurium i.p). 7 days after infection, mice intestines were collected and total number of bacteria were counted. Data were analyzed using One-Way ANOVA and Post Hoct test. The result shows ANOVA p-value = 0.006 and p-value of Post Hoc = 0.004, proving that black seed oil has potential as immunomodulator in mice infected with Salmonella typhimurium.

Paper Citation

in Harvard Style

Wulandari E., Krihariyani D. and Suliati. (2018). Potential of Black Seed Oil (Nigella Sativa) as Immunomodulator in Mice (Mus Musculus) Infected with Salmonella Typhimurium.In Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS, ISBN 978-989-758-348-3, pages 407-411. DOI: 10.5220/0007543904070411

in Bibtex Style

@conference{icps18,
author={Eka Yunita Wulandari and Dwi Krihariyani and Suliati},
title={Potential of Black Seed Oil (Nigella Sativa) as Immunomodulator in Mice (Mus Musculus) Infected with Salmonella Typhimurium},
booktitle={Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,},
year={2018},
pages={407-411},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007543904070411},
isbn={978-989-758-348-3},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,
TI - Potential of Black Seed Oil (Nigella Sativa) as Immunomodulator in Mice (Mus Musculus) Infected with Salmonella Typhimurium
SN - 978-989-758-348-3
AU - Wulandari E.
AU - Krihariyani D.
AU - Suliati.
PY - 2018
SP - 407
EP - 411
DO - 10.5220/0007543904070411