The Potential of Antigenic Protein of Sarcoptes scabiei as a Serological Diagnostic Candidate for Scabies in Goats

Nunuk Dyah Retno Lastuti, Dony Chrismanto, Poedji Hastutiek, Agus Sunarso

2018

Abstract

In Indonesia, the prevalence of scabies on goat cattle is still high, which shows that scabies is still not handled properly. It is now considered as an emerging/re-emerging parasitic disease that threatens human and animal health globally. The scabies disease has been known for a thousand years, and it is a persistent problem on public health and also livestock. Yet, until today a serological diagnostic tool is sensitive and specific is not available, especially for goat cattle in Indonesia. In recent years, an indirect antibody enzyme-linked immunosorbent assay (ELISA) has been available, which has higher sensitivity and specificity than traditional diagnostic methods. In order to overcome those problems preventive actions need to be undertaken through research for serological diagnostic development as an alternative for scabies prevention in goats in Indonesia. The purpose of this research is doing sensitivity and specificity tests towards the antigenic protein of Sarcoptes scabiei isolated from goat as diagnostic material for scabies in goats. The method was comprised of deciding the gold standard for positive and negative scabies, checkerboard test toward antigenic protein, and ELISA test for measuring sensitivity and specificity. The research results showed that S. scabiei antigenic protein with molecular weight of 57.3 kDa can be recognized by serum antibody of goat that infested with scabies with sensitivity level of 96% and specificity of 86,6%. From that result, it can be recommended that S.scabiei with a molecular weight of 57.3 kDa is the specific antigenic protein that can be used as a candidate of serological diagnostic material for scabies isolated from Indonesian local goat.

Paper Citation

in Harvard Style

Lastuti N., Chrismanto D., Hastutiek P. and Sunarso A. (2018). The Potential of Antigenic Protein of Sarcoptes scabiei as a Serological Diagnostic Candidate for Scabies in Goats.In Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS, ISBN 978-989-758-348-3, pages 537-540. DOI: 10.5220/0007546505370540

in Bibtex Style

@conference{icps18,
author={Nunuk Dyah Retno Lastuti and Dony Chrismanto and Poedji Hastutiek and Agus Sunarso},
title={The Potential of Antigenic Protein of Sarcoptes scabiei as a Serological Diagnostic Candidate for Scabies in Goats},
booktitle={Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,},
year={2018},
pages={537-540},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007546505370540},
isbn={978-989-758-348-3},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,
TI - The Potential of Antigenic Protein of Sarcoptes scabiei as a Serological Diagnostic Candidate for Scabies in Goats
SN - 978-989-758-348-3
AU - Lastuti N.
AU - Chrismanto D.
AU - Hastutiek P.
AU - Sunarso A.
PY - 2018
SP - 537
EP - 540
DO - 10.5220/0007546505370540