The Morphology of Contaminant Organism in Kappaphycus alvarezii Tissue Culture

Ulfatus Zahroh, Apri Arisandi

2018

Abstract

The main problem in increasing the production of seaweed cultivation of Kappaphycus alvarezii is the availability of quality seeds. One of the causes is because the seeds are susceptible to infectious diseases. Tissue culture is one of the techniques to produce Specific Pathogen and Epiphyte Free /SPE Nevertheless, the presence or absence of contamination needs to be analyzed to determine the cause of contamination,, morphology of contaminating organisms, and changes of morphological cell of tissue culture which can be used to prevent contamination during the next tissue culture and cultivation at the sea. Based on the results, it could be revealed that the occurring contamination caused by bacteria and fungi as well as caused by the less sterill culture process. Thallus morphology affected by the disease has slower growth. There were also black spots, cotton-like substance as contaminated fungi (Saprolegnia sp and phytopthora), and the fading of green and slimy pigment as bacteria contamination. In addition, the morpology of ill seaweed cells has smaller cells and shrinked tissue compared to the healthy ones with their bigger cells and no shrinkage.

Paper Citation

in Harvard Style

Zahroh U. and Arisandi A. (2018). The Morphology of Contaminant Organism in Kappaphycus alvarezii Tissue Culture.In Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS, ISBN 978-989-758-348-3, pages 587-594. DOI: 10.5220/0007547605870594

in Bibtex Style

@conference{icps18,
author={Ulfatus Zahroh and Apri Arisandi},
title={The Morphology of Contaminant Organism in Kappaphycus alvarezii Tissue Culture},
booktitle={Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,},
year={2018},
pages={587-594},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007547605870594},
isbn={978-989-758-348-3},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,
TI - The Morphology of Contaminant Organism in Kappaphycus alvarezii Tissue Culture
SN - 978-989-758-348-3
AU - Zahroh U.
AU - Arisandi A.
PY - 2018
SP - 587
EP - 594
DO - 10.5220/0007547605870594