Molecular Characterization and Antibiotic Resistance of Yersinia Entrocolitica Strains Isolated from Fish

X. L. Gou, J. Li, L. Wang, H. L. Zhao, Y. Q. Su

2018

Abstract

Yersinia enterocolitica, a widespread food and water-borne pathogen, is responsible for diseases in humans and animals. The aims of this study were to determine the molecular epidemiology of Y. enterocolitica strains isolated from, as well as, to investigate their pathogenic potential and assess the antibiotic resistance. Twelve Y. enterocolitica strains isolated from diseased fish were studied. Genotypic diversity was analyzed by ERIC-PCR and 16S rDNA-RFLP, virulence genes were assessed by PCR method. Kirby-Bauer disk diffusion method was applied for the antibiotic resistance profile. The results indicated that (i) The 12 strains were grouped into five clusters by ERIC-PCR with similarity ranging from 81.8% to 100%, while four RFLP types were identified by 16S rDNA-RFLP with similarity ranging from 83.3% to 100%. (ii) Genes ail and intB were detected in all strains, whereas, virF and ystB were found at high probability of 83.3%, 75%, respectively, yadA was 41.6%. (iii) The isolated strains showed antibiotics resistance as follows, lincomycin (100%), sulfafurazole (41.6%), cephalothin V (66.7%), rifampicin (75.0%) compound sulfamethoxazole (8.3%), streptomycin (8.3%) and rocephin (8.3%), but all were sensitive to gentamicin, kanamycin, tetracycline and enrofloxacin. It will benefit the further study of pathogenesis and prevention of Y. enterocolitica in.

Paper Citation

in Harvard Style

Gou X., Li J., Wang L., Zhao H. and Su Y. (2018). Molecular Characterization and Antibiotic Resistance of Yersinia Entrocolitica Strains Isolated from Fish.In Proceedings of the International Workshop on Environmental Management, Science and Engineering - Volume 1: IWEMSE, ISBN 978-989-758-344-5, pages 654-662. DOI: 10.5220/0007564206540662

in Bibtex Style

@conference{iwemse18,
author={X. L. Gou and J. Li and L. Wang and H. L. Zhao and Y. Q. Su},
title={Molecular Characterization and Antibiotic Resistance of Yersinia Entrocolitica Strains Isolated from Fish},
booktitle={Proceedings of the International Workshop on Environmental Management, Science and Engineering - Volume 1: IWEMSE,},
year={2018},
pages={654-662},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007564206540662},
isbn={978-989-758-344-5},
}

in EndNote Style

TY - CONF

JO - Proceedings of the International Workshop on Environmental Management, Science and Engineering - Volume 1: IWEMSE,
TI - Molecular Characterization and Antibiotic Resistance of Yersinia Entrocolitica Strains Isolated from Fish
SN - 978-989-758-344-5
AU - Gou X.
AU - Li J.
AU - Wang L.
AU - Zhao H.
AU - Su Y.
PY - 2018
SP - 654
EP - 662
DO - 10.5220/0007564206540662