The Effect of Mesenchymal Stem Cell Conditioned Medium (Adipose-Derived and Wharton's Jelly-Derived) on the Prevention of Hypertrophic Scar Formation

Gita Hening Bunga, Retno Dwi Utami, Erlina Pricilla Sitorus, Novan Adi Setyawan, Indah Julianto, Moerbono Mochtar

2018

Abstract

The process of wound healing can lead to the formation of varied outcomes, from scarless healing to excessive fibrosis (hypertrophic) or atrophic scar. Mesenchymal stem cell (MSC) had been shown to prevent the growth of fibrosis tissue. Conditioned media (CM) is the medium where stem cells are cultured. Adipose-Derived Stem Cell Conditioned Medium (ADSC-CM) and Wharton’s Jelly Stem Cell Conditioned Medium (WJSC-CM) are MSC-CM reported to contain growth factors, some of which plays a role in the formation of hypertrophic scar such as TGFβ1 and VEGF, and some in the prevention of scar formation, such as bFGF. This in vivo study conducted using 24 mice, divided into 4 groups: group I given Dulbecco's Modified Eagle Medium (DMEM), group II ADSC-CM, group III WJSC-CM, and group IV without any treatment (control). Before the injection, wound was made on the back of each mice with a 1 cm punch biopsy. On day 28th, rebiopsy was done on the scar area, the tissue stained with hematoxyllin eosin staining to assess the Scar Elevation Index (SEI). The SEI score showed that WJSC-CM group showed the lowest percentage of hypertrophic scar formation (50%) compared to ADSC-CM (66.67%), DMEM (83.33%) or control group(100%), although statistically the difference was not significant. This study showed that WJSC-CM injection had a greater potential in preventing the formation of hypertrophic scar than ADSC- CM. It is thought to be related to higher bFGF levels as well as lower TGFβ1 in WJSC-CM.

Paper Citation

in Harvard Style

Bunga G., Utami R., Sitorus E., Setyawan N., Julianto I. and Mochtar M. (2018). The Effect of Mesenchymal Stem Cell Conditioned Medium (Adipose-Derived and Wharton's Jelly-Derived) on the Prevention of Hypertrophic Scar Formation.In Proceedings of the 23rd Regional Conference of Dermatology - Volume 1: RCD, ISBN 978-989-758-494-7, pages 146-149. DOI: 10.5220/0008152701460149

in Bibtex Style

@conference{rcd18,
author={Gita Hening Bunga and Retno Dwi Utami and Erlina Pricilla Sitorus and Novan Adi Setyawan and Indah Julianto and Moerbono Mochtar},
title={The Effect of Mesenchymal Stem Cell Conditioned Medium (Adipose-Derived and Wharton's Jelly-Derived) on the Prevention of Hypertrophic Scar Formation},
booktitle={Proceedings of the 23rd Regional Conference of Dermatology - Volume 1: RCD,},
year={2018},
pages={146-149},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008152701460149},
isbn={978-989-758-494-7},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 23rd Regional Conference of Dermatology - Volume 1: RCD,
TI - The Effect of Mesenchymal Stem Cell Conditioned Medium (Adipose-Derived and Wharton's Jelly-Derived) on the Prevention of Hypertrophic Scar Formation
SN - 978-989-758-494-7
AU - Bunga G.
AU - Utami R.
AU - Sitorus E.
AU - Setyawan N.
AU - Julianto I.
AU - Mochtar M.
PY - 2018
SP - 146
EP - 149
DO - 10.5220/0008152701460149