The Effect of Advanced Adipose-derived Stem Cell Protein Extract to Repairment of Collagen Deposition in Cultured Senescent Fibroblast

Erlina Pricilla Sitorus, Retno Dwi Utami, Gita Hening Bunga, Indah Julianto, Harijono Kariosentono, Chandrani Khoirinaya, Brian Wasita

2018

Abstract

Aging skin is progressive process in skin that stimulates by environment damage and influences the appearance. There are two factors inducing aging, intrinsic i.e genetic and age, and extrinsic i.e sun expose, air pollutant, smoking, alcoholic and malnutrition. Recently, cell based therapy has been widely reported as the option for aging skin therapy. Advanced adipose-derived stem cell protein extract (AAPE) is a conditioned medium cultured under a hypoxia of Adipose-derived stem cell (ADSC), which can stimulate collagen synthesis and migration dermal fibroblast, thus induces wound healing and wrinkles repairment. This study samples used cultured fibroblast from amnion, with donor from a 30 year old woman by cesarean section on her first delivery. There were three samples cultured groups, normal fibroblast (control), normal fibroblast with 30 minutes UVB exposure in basal media (study group 1), and normal fibroblast with 30 minutes UVB exposure in basal media added AAPE (study group 2). All groups were evaluated in 48 hours and checked the collagen density by using immunocytochemistry assay with collagen antibody-1. The results were interpreted by Image-J software. Study group 2 showed significant increasing of collagen density, with p value 0.001. Because the AAPE was derived from ADSC thus it has the same growth factors as those of ADSC. AAPE had been proven in our study that it stimulated collagen deposition.

Paper Citation

in Harvard Style

Sitorus E., Utami R., Bunga G., Julianto I., Kariosentono H., Khoirinaya C. and Wasita B. (2018). The Effect of Advanced Adipose-derived Stem Cell Protein Extract to Repairment of Collagen Deposition in Cultured Senescent Fibroblast.In Proceedings of the 23rd Regional Conference of Dermatology - Volume 1: RCD, ISBN 978-989-758-494-7, pages 272-275. DOI: 10.5220/0008155502720275

in Bibtex Style

@conference{rcd18,
author={Erlina Pricilla Sitorus and Retno Dwi Utami and Gita Hening Bunga and Indah Julianto and Harijono Kariosentono and Chandrani Khoirinaya and Brian Wasita},
title={The Effect of Advanced Adipose-derived Stem Cell Protein Extract to Repairment of Collagen Deposition in Cultured Senescent Fibroblast},
booktitle={Proceedings of the 23rd Regional Conference of Dermatology - Volume 1: RCD,},
year={2018},
pages={272-275},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008155502720275},
isbn={978-989-758-494-7},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 23rd Regional Conference of Dermatology - Volume 1: RCD,
TI - The Effect of Advanced Adipose-derived Stem Cell Protein Extract to Repairment of Collagen Deposition in Cultured Senescent Fibroblast
SN - 978-989-758-494-7
AU - Sitorus E.
AU - Utami R.
AU - Bunga G.
AU - Julianto I.
AU - Kariosentono H.
AU - Khoirinaya C.
AU - Wasita B.
PY - 2018
SP - 272
EP - 275
DO - 10.5220/0008155502720275