The Correlation between Neutrophil CD64, Interleukin-17 (Il-17), Interleukin-10 (Il-10) in Skin Tissue and Neutrophil CD64, Il-17 and Il-10 in Blood Circulation in Erythema Nodosum Leprosum (ENL) Patients

I. Gusti Nyoman Darmaputra, Luh Mas Rusyati, Wibi Riawan, Anang Endaryanto, Cita Rosita Sigit Prakoeswa

2018

Abstract

Erythema nodosum leprosum (ENL) remain major health problem globally. Current pathogenesis of ENL not only involving immune complex, but also neutrophil and cellular immune response. Neutrophil CD64, interleukin-17 (IL-17) and interleukin-10 (IL-10) are mediators that suggested to be involved in regulation of ENL. This study aimed to determine the correlation between neutrophil CD64, IL-17 and IL-10 in skin tissue with neutrophil CD64, IL-17 and IL-10 in blood circulation. This was an analytical observational study with a cross-sectional design which involved 30 patients of multibacillary (MB) type of leprosy patients which underwent ENL. Neutrophil CD64 levels in blood circulation were measured by flow cytometry, while IL-10 and IL-17 levels in the blood circulation with ELISA. Biopsy was performed prior to the examination of neutrophil CD64, IL-17 and IL-10 from skin tissue with immunohistochemistry (IHC). The obtained data were analyzed descriptively and with correlation analysis. The mean of neutrophil CD64, IL-17 and IL-10 in skin tissue was 19.10 ± 5.371, 11.47 ± 5.029 and 6.73 ± 3.039, respectively, while the mean of neutrophil CD 64, IL-17 and IL-10 in circulation was 66.807 ± 14.794, 59.55 ± 36.397 and 186.30 ± 272.974, respectively. No significant correlation was found between IL-17, neutrophil CD64 and IL-10 in skin tissue with IL- 17, neutrophil CD64 and IL-10 in blood circulation with significance level of 0.511, 0.186 and 0.401, respectively. There was no correlation between dysregulation of local immunity with systemic immunity in ENL.

Paper Citation

in Harvard Style

Darmaputra I., Rusyati L., Riawan W., Endaryanto A. and Prakoeswa C. (2018). The Correlation between Neutrophil CD64, Interleukin-17 (Il-17), Interleukin-10 (Il-10) in Skin Tissue and Neutrophil CD64, Il-17 and Il-10 in Blood Circulation in Erythema Nodosum Leprosum (ENL) Patients.In Proceedings of the 23rd Regional Conference of Dermatology - Volume 1: RCD, ISBN 978-989-758-494-7, pages 286-290. DOI: 10.5220/0008155802860290

in Bibtex Style

@conference{rcd18,
author={I. Gusti Nyoman Darmaputra and Luh Mas Rusyati and Wibi Riawan and Anang Endaryanto and Cita Rosita Sigit Prakoeswa},
title={The Correlation between Neutrophil CD64, Interleukin-17 (Il-17), Interleukin-10 (Il-10) in Skin Tissue and Neutrophil CD64, Il-17 and Il-10 in Blood Circulation in Erythema Nodosum Leprosum (ENL) Patients},
booktitle={Proceedings of the 23rd Regional Conference of Dermatology - Volume 1: RCD,},
year={2018},
pages={286-290},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008155802860290},
isbn={978-989-758-494-7},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 23rd Regional Conference of Dermatology - Volume 1: RCD,
TI - The Correlation between Neutrophil CD64, Interleukin-17 (Il-17), Interleukin-10 (Il-10) in Skin Tissue and Neutrophil CD64, Il-17 and Il-10 in Blood Circulation in Erythema Nodosum Leprosum (ENL) Patients
SN - 978-989-758-494-7
AU - Darmaputra I.
AU - Rusyati L.
AU - Riawan W.
AU - Endaryanto A.
AU - Prakoeswa C.
PY - 2018
SP - 286
EP - 290
DO - 10.5220/0008155802860290