Serological Antibody Profile IgM and IgG of Mycobacterium leprae PGL-1 and L-ESAT-6 in Patients and Household Contact from Leprosy Endemic Area in East Java Indonesia

A. Dinar Adriaty, M. Irfan Hadi, M. Irfan Hadi, Irmadita Citrashanty, Iswahyudi , Indropo Agusni, Indropo Agusni, Shinzo Izumi, Cita S. P.

2018

Abstract

Leprosy patients in Indonesia is the third largest in the world. The problem of high transmission is the difficulty of early detection of leprosy. At present, diagnosis leprosy still based on clinical sign. Some of supporting diagnostic tools are developed, such as serological test. The most widely used antigen for diagnostics is Phenolic-glycolipid-1(PGL-1), but some limitations of the antigen, provide a challenge to find a potential candidate antigen representing specific Mycobacterium leprae. Purpose of this research is for studying Mycobacterium leprae L-ESAT-6 (epitope AA11-36) compare to PGL- 1 to leprosy patients and household contacts in leprosy endemic region. Analysis have been conducted to MB and PB leprosy patients, as well as their families with total 173 respondents by testing Indirect ELISA of the L-ESAT-6 and PGL-1. In general, the profile of ELISA test anti PGL-1 vs L-ESAT-6 in all the patients don’t have significant difference, but in household contacts, with the Pearson correlation test, it can be concluded that there are significant difference between PGL-1 and L-ESAT-6 (p-value = 0.049; p <α = 0.05). Healthy individuals who are exposed to M.leprae found high titers of antibody anti L-ESAT-6 and lower profile of antibody anti PGL-1. It is assumed that the individual is relatively immune to M.leprae. So it can be concluded that L-ESAT-6 (AA11-36) can be used as candidate diagnostic test which is a predictor marker for people living in endemic leprosy areas, for it still need a further research.

Paper Citation

in Harvard Style

Adriaty A., Hadi M., Citrashanty I., I., Agusni I., Izumi S. and S. P. C. (2018). Serological Antibody Profile IgM and IgG of Mycobacterium leprae PGL-1 and L-ESAT-6 in Patients and Household Contact from Leprosy Endemic Area in East Java Indonesia.In Proceedings of the 23rd Regional Conference of Dermatology - Volume 1: RCD, ISBN 978-989-758-494-7, pages 323-327. DOI: 10.5220/0008156703230327

in Bibtex Style

@conference{rcd18,
author={A. Dinar Adriaty and M. Irfan Hadi and Irmadita Citrashanty and Iswahyudi and Indropo Agusni and Shinzo Izumi and Cita S. P.},
title={Serological Antibody Profile IgM and IgG of Mycobacterium leprae PGL-1 and L-ESAT-6 in Patients and Household Contact from Leprosy Endemic Area in East Java Indonesia},
booktitle={Proceedings of the 23rd Regional Conference of Dermatology - Volume 1: RCD,},
year={2018},
pages={323-327},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008156703230327},
isbn={978-989-758-494-7},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 23rd Regional Conference of Dermatology - Volume 1: RCD,
TI - Serological Antibody Profile IgM and IgG of Mycobacterium leprae PGL-1 and L-ESAT-6 in Patients and Household Contact from Leprosy Endemic Area in East Java Indonesia
SN - 978-989-758-494-7
AU - Adriaty A.
AU - Hadi M.
AU - Citrashanty I.
AU - I.
AU - Agusni I.
AU - Izumi S.
AU - S. P. C.
PY - 2018
SP - 323
EP - 327
DO - 10.5220/0008156703230327