Mutation of Beauveria Bassiana Using Low-energy N+ Implantation and Selection of a High Virulence Strain

Xiaojun Deng, Jian Tang, Guoying Zhou, Jizhao Cao

2018

Abstract

Low-energy ion implantation technology was used to generate mutants of the high virulence Beauveria bassiana strain BbIII and bioassays were carried out on Biston marginata. The survival rate curve of B. bassiana to doses of N+ showed a “saddle shape.” The peak value 15 × 1014 ions·cm-2 was the optimal dose for mutagenesis. The growth characteristics of B. bassiana, such as colony morphology, sporulation, and spore germination rate, were affected by N+ implantation. Three stains of B. bassiana were selected based on sporulation, chitinase, and Pr1 enzyme activity as the screening indices. The activities of chitinase and Pr1 enzyme in Bb III 22 were 0.230 and 0.137 (OD value), and these were almost twice that of the original strain. The Bb III 22 strain was the most virulent to B. marginata and the mortality was > 86.7% at a concentration of 107 spores·mL-1. The log LD50 of the mutant strain spores against B. marginata was 5.1951 (4.5174~5.8416). The results indicate that low-energy N+ implantation and mutation can be effective for increasing the virulence of Beauveria bassiana.

Paper Citation

in Harvard Style

Deng X., Tang J., Zhou G. and Cao J. (2018). Mutation of Beauveria Bassiana Using Low-energy N+ Implantation and Selection of a High Virulence Strain.In The Second International Conference on Materials Chemistry and Environmental Protection - MEEP, ISBN 978-989-758-360-5, pages 19-24. DOI: 10.5220/0008184800190024

in Bibtex Style

@conference{meep18,
author={Xiaojun Deng and Jian Tang and Guoying Zhou and Jizhao Cao},
title={Mutation of Beauveria Bassiana Using Low-energy N+ Implantation and Selection of a High Virulence Strain},
booktitle={The Second International Conference on Materials Chemistry and Environmental Protection - MEEP,},
year={2018},
pages={19-24},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008184800190024},
isbn={978-989-758-360-5},
}

in EndNote Style

TY - CONF

JO - The Second International Conference on Materials Chemistry and Environmental Protection - MEEP,
TI - Mutation of Beauveria Bassiana Using Low-energy N+ Implantation and Selection of a High Virulence Strain
SN - 978-989-758-360-5
AU - Deng X.
AU - Tang J.
AU - Zhou G.
AU - Cao J.
PY - 2018
SP - 19
EP - 24
DO - 10.5220/0008184800190024