Establishment of a Rapid and Sensitive Chemiluminescence Enzyme Immunoasay for Aflatoxin M1: Verified by HPLC

Min Li, Renrong Liu, Lixin Zhu, Yu Jin

2018

Abstract

A rapid and sensitive chemiluminescence enzyme immunoassay method (CLEIA) was established to detect Aflatoxin M1 (AFM1) in milk, which was Verified by high performance liquid chromatography (HPLC). It only takes 30 minutes. Optimized conditions included antibody dilution ratio and enzyme conjugate, ionic strength, pH value and organic solvent. Results: The 50% inhibitory concentration(IC50) and the detection limit of the CLEIA were 0.08ng/mL and 0.024ng/mL, the recovery ranged from 86.94% to 114.49% in dairy products. The correlation was 99.7% between this method and HPLC .

Paper Citation

in Harvard Style

Li M., Liu R., Zhu L. and Jin Y. (2018). Establishment of a Rapid and Sensitive Chemiluminescence Enzyme Immunoasay for Aflatoxin M1: Verified by HPLC.In The Second International Conference on Materials Chemistry and Environmental Protection - MEEP, ISBN 978-989-758-360-5, pages 51-54. DOI: 10.5220/0008185300510054

in Bibtex Style

@conference{meep18,
author={Min Li and Renrong Liu and Lixin Zhu and Yu Jin},
title={Establishment of a Rapid and Sensitive Chemiluminescence Enzyme Immunoasay for Aflatoxin M1: Verified by HPLC},
booktitle={The Second International Conference on Materials Chemistry and Environmental Protection - MEEP,},
year={2018},
pages={51-54},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008185300510054},
isbn={978-989-758-360-5},
}

in EndNote Style

TY - CONF

JO - The Second International Conference on Materials Chemistry and Environmental Protection - MEEP,
TI - Establishment of a Rapid and Sensitive Chemiluminescence Enzyme Immunoasay for Aflatoxin M1: Verified by HPLC
SN - 978-989-758-360-5
AU - Li M.
AU - Liu R.
AU - Zhu L.
AU - Jin Y.
PY - 2018
SP - 51
EP - 54
DO - 10.5220/0008185300510054