Determination of Phorate and Its Metabolite Phorate Sulfone Residues in the Egg by High Performance Liquid Chromatography-tandem Mass Spectrometry

Hongbin Guo, Jianzhi Ye, Yuanjing Liu, Ling Lin, Chunliang Yang, Qi Li

2018

Abstract

In this study, we present a newly developed fast and sensitive method for the quantification of phorate and its metabolite phorate sulfone residues in the egg, using solid phase extraction and UPLC-MS/MS analysis.The eggs sample were extracted with acetonitrile under neutral conditions in this paper , cleaned up by the glass funnel plugged with cotton and salts equipped with anhydrous sodium sulfate and neutral alumina, and determinatied by waters high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). There are good linear correlations between the peak area and the corresponding concentration of phorate and its metabolite phorate sulfone in the range of 2.00-100.00 ng/mL and the linear coefficient of the three pesticides is above 0.9995. The recovery rate of phorate and its metabolite phorate sulfone ranged from 78.6% to 95.6%, and with the batch relative deviation ranged from 0.3% to 5.6%. The lower limit detection (LOD) of the phorate sulfone, phorate and phorate sulfoxide were 0.0005 mg/kg , 0.0005mg/kg and 0.0005 mg/kg, respectively. The phorate, phorate sulfone and phorate sulfoxide's quantitative（LOQ）were 0.0015 mg/kg, 0.0015 mg/kg and 0.0015 mg/kg, respectively. The results show that UPLC-MS/MS in conjunction with phorate and its metabolite phorate sulfone is a powerful tool for screening the inhibitory effects of eggs and can thus be used for safety control in food processing procedures.

Paper Citation

in Harvard Style

Guo H., Ye J., Liu Y., Lin L., Yang C. and Li Q. (2018). Determination of Phorate and Its Metabolite Phorate Sulfone Residues in the Egg by High Performance Liquid Chromatography-tandem Mass Spectrometry.In The Second International Conference on Materials Chemistry and Environmental Protection - MEEP, ISBN 978-989-758-360-5, pages 63-68. DOI: 10.5220/0008185600630068

in Bibtex Style

@conference{meep18,
author={Hongbin Guo and Jianzhi Ye and Yuanjing Liu and Ling Lin and Chunliang Yang and Qi Li},
title={Determination of Phorate and Its Metabolite Phorate Sulfone Residues in the Egg by High Performance Liquid Chromatography-tandem Mass Spectrometry},
booktitle={The Second International Conference on Materials Chemistry and Environmental Protection - MEEP,},
year={2018},
pages={63-68},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008185600630068},
isbn={978-989-758-360-5},
}

in EndNote Style

TY - CONF

JO - The Second International Conference on Materials Chemistry and Environmental Protection - MEEP,
TI - Determination of Phorate and Its Metabolite Phorate Sulfone Residues in the Egg by High Performance Liquid Chromatography-tandem Mass Spectrometry
SN - 978-989-758-360-5
AU - Guo H.
AU - Ye J.
AU - Liu Y.
AU - Lin L.
AU - Yang C.
AU - Li Q.
PY - 2018
SP - 63
EP - 68
DO - 10.5220/0008185600630068