Immobilization of Horseradish Peroxidase on Modified Electrospun Nanofibrous Membrane for 2,4-Dichlorophenol Removal

Ning Wei, Ran Xu, Rongzhi Tang

2018

Abstract

In this study, a facile and simple method was used to modify the hydrophobic PAN/PVdF membranes into hydrophilic ones. Results showed that the PAN/PVdF membranes have been modified successfully and the water contact angle changed from 87.135° to 0°. Horseradish peroxidase (HRP) was immobilized onto the modified PAN/PVdF membranes through covalent binding and the maximum enzyme loading was approximately 440 mg/g under optimal conditions (after 8 h at pH 8.0 and 25 oC). The effects of pH and temperature on the relative activity of free and immobilized HRP were studied. Under the optimum conditions of pH and temperature, immobilized HRP was greatly improved in operational and storage stability. 2,4-DCP removal experiments showed that the immobilized HRP and free HRP had a similar removal efficiency (87% and 93%, respectively). However, the immobilized HRP had an excellent reusability. HRP-PAN/PVdF could still remove 47.6% of 2,4-DCP after 7 repeated runs, which could overcome the inherent drawbacks of free enzymes-hard separation and non-reusability.

Paper Citation

in Harvard Style

Wei N., Xu R. and Tang R. (2018). Immobilization of Horseradish Peroxidase on Modified Electrospun Nanofibrous Membrane for 2,4-Dichlorophenol Removal.In The Second International Conference on Materials Chemistry and Environmental Protection - MEEP, ISBN 978-989-758-360-5, pages 283-292. DOI: 10.5220/0008188902830292

in Bibtex Style

@conference{meep18,
author={Ning Wei and Ran Xu and Rongzhi Tang},
title={Immobilization of Horseradish Peroxidase on Modified Electrospun Nanofibrous Membrane for 2,4-Dichlorophenol Removal},
booktitle={The Second International Conference on Materials Chemistry and Environmental Protection - MEEP,},
year={2018},
pages={283-292},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008188902830292},
isbn={978-989-758-360-5},
}

in EndNote Style

TY - CONF

JO - The Second International Conference on Materials Chemistry and Environmental Protection - MEEP,
TI - Immobilization of Horseradish Peroxidase on Modified Electrospun Nanofibrous Membrane for 2,4-Dichlorophenol Removal
SN - 978-989-758-360-5
AU - Wei N.
AU - Xu R.
AU - Tang R.
PY - 2018
SP - 283
EP - 292
DO - 10.5220/0008188902830292