Pre-stimulation of Bone-marrow Derived Eosinophils with CCL24 Alters Responses to TLR Ligands and Helminth Extracts

B. C. Buerfent, A. Ehrens, W. Stamminger, A. Hoerauf, M. P. Hübner

2018

Abstract

Eosinophil granulocytes are a hallmark of helminth infection, provide protection against helminth infections and elicit detrimental effects during allergy and asthma. Moreover, eosinophils are associated with diabetes, arthritis and sepsis. Thus, eosinophils have a broad range of implications, contributing to homeostasis as well as pathogenesis of various diseases. In the current study we used murine bone-marrow derived eosinophils (bmEos) to investigate the impact of eosinophil pre-stimulation with the chemoattractants CCL11 and CCL24 (eotaxin-1 and 2) on TLR2, TLR4 and filarial extract-induced eosinophil responses. Generation of bmEos consistently resulted in approximately 50 million bmEos from a single donor mouse and a purity and viability above 95%. Upon stimulation with CCL24, TLR2, TLR4, and filarial extract, bmEos released different quantities of IL-4, IL-6, CCL5, as well as CXCL1. CCL24 pre-stimulation partially affected those responses. Furthermore, CCL24 pre-stimulation of bmEos reduced the expression of the eotaxin receptor CCR3 independently of TLR2 stimulation. In contrast, expression of adhesion molecule ICAM-1 was increased by TLR2 stimulation, but not affected by CCL24 pre-stimulation. Hence, our results reveal an impact of CCL24 on bmEos activation. bmEos present a promising tool to study eosinophil responses that may help to further characterize their role in different immunological contexts and overcome the limitations given by the low eosinophil frequencies present in non-helminth-infected individuals.

Paper Citation

in Harvard Style

Buerfent B., Ehrens A., Stamminger W., Hoerauf A. and Hübner M. (2018). Pre-stimulation of Bone-marrow Derived Eosinophils with CCL24 Alters Responses to TLR Ligands and Helminth Extracts.In Proceedings of the 2nd Syiah Kuala International Conference on Medicine and Health Sciences - Volume 1: SKIC-MHS, ISBN 978-989-758-438-1, pages 5-9. DOI: 10.5220/0008787800050009

in Bibtex Style

@conference{skic-mhs18,
author={B. C. Buerfent and A. Ehrens and W. Stamminger and A. Hoerauf and M. P. Hübner},
title={Pre-stimulation of Bone-marrow Derived Eosinophils with CCL24 Alters Responses to TLR Ligands and Helminth Extracts},
booktitle={Proceedings of the 2nd Syiah Kuala International Conference on Medicine and Health Sciences - Volume 1: SKIC-MHS,},
year={2018},
pages={5-9},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008787800050009},
isbn={978-989-758-438-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd Syiah Kuala International Conference on Medicine and Health Sciences - Volume 1: SKIC-MHS,
TI - Pre-stimulation of Bone-marrow Derived Eosinophils with CCL24 Alters Responses to TLR Ligands and Helminth Extracts
SN - 978-989-758-438-1
AU - Buerfent B.
AU - Ehrens A.
AU - Stamminger W.
AU - Hoerauf A.
AU - Hübner M.
PY - 2018
SP - 5
EP - 9
DO - 10.5220/0008787800050009