Protective Effect of Neem (Azadirachta Indica) Leaf Extract on Liver of Trypanosoma evansi Infected Rats (Rattus norvegicus)

Yudha Fahrimal, Dwina Aliza, Erina, Ayu Dwi Fitriani, Al Azhar

2018

Abstract

Animal trypanosomiasis is still a devastating disease of both domestic and wild animals around the world. Control of surra is greatly depending on chemotherapy. The aim of this research was to observe histological changes in the liver of T.evansi infected rats treated with different concentrations of neem (Azadirachta indica) leaf extract. Samples used were livers collected from 24 male Wistar rats randomly divided into 6 treatment groups with 4 replications each. Negative control (K0) was rats given distilled water only; positive control (K1) was rats infected with T.evansi 5x104; K2, K3, K4, and K5 were rats infected with T. evansi 5x104 and administered with neem leaf extract 50, 100, 400, and 800 mg/kg body weight (BW) per oral for 3 consecutive days, respectively. On day 4, all rats were sacrificed by ether euthanation for liver collection. Livers obtained were histopathologically processed using standard hematoxylin-eosin staining and microscopically observed. Histological changes in the liver of rats in all treatment groups were as the following. Normal hepatocytes were K0 63.65, K1 27.25, K2 15.15, K3 12.90, K4 20.30, and K5 24.85.Haemorrhagic hepatocytes were K0 0.00, K1 15.60, K2 10.60, K3 17.75, K4 6.30 and K5 9.05. Hyperemic hepatocytes were K0 0.00, K1 2.35, K2 3.70, K3 3.25, K4 2.25 and K5 2.25. Infiltration of inflamation cells were K0 0.00, K1 2.75, K2 3.90, K3 14.65, K4 2.55, and K5 2.70. Hepatocyte degeneration was K0 0.00, K1 6.30, K2 6.95, K3 10.15, K4 4.15, and and K5 1.00. Necrotic hepatocytes were K0 1.55, K1 32.20, K2 43.80, K3 45.00, K4 31.25, and K534.20. Neem leaf extract at the dose of 800 mg/kg BW was the best in preventing liver damages caused by T. evansi infection in rats.

Paper Citation

in Harvard Style

Fahrimal Y., Aliza D., Erina., Fitriani A. and Azhar A. (2018). Protective Effect of Neem (Azadirachta Indica) Leaf Extract on Liver of Trypanosoma evansi Infected Rats (Rattus norvegicus).In Proceedings of the 2nd Syiah Kuala International Conference on Medicine and Health Sciences - Volume 1: SKIC-MHS, ISBN 978-989-758-438-1, pages 172-177. DOI: 10.5220/0008788401720177

in Bibtex Style

@conference{skic-mhs18,
author={Yudha Fahrimal and Dwina Aliza and Erina and Ayu Dwi Fitriani and Al Azhar},
title={Protective Effect of Neem (Azadirachta Indica) Leaf Extract on Liver of Trypanosoma evansi Infected Rats (Rattus norvegicus)},
booktitle={Proceedings of the 2nd Syiah Kuala International Conference on Medicine and Health Sciences - Volume 1: SKIC-MHS,},
year={2018},
pages={172-177},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008788401720177},
isbn={978-989-758-438-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd Syiah Kuala International Conference on Medicine and Health Sciences - Volume 1: SKIC-MHS,
TI - Protective Effect of Neem (Azadirachta Indica) Leaf Extract on Liver of Trypanosoma evansi Infected Rats (Rattus norvegicus)
SN - 978-989-758-438-1
AU - Fahrimal Y.
AU - Aliza D.
AU - Erina.
AU - Fitriani A.
AU - Azhar A.
PY - 2018
SP - 172
EP - 177
DO - 10.5220/0008788401720177