Antibacterial Activity of Methanol Extract of Calotropis gigantea Flowers from Aceh

Kartini Hasballah

2018

Abstract

The purpose of this study was to determine the antibacterial activity of methanol extract of Calotropis gigantea flowers at various concentrations of gram-positive bacteria (Staphylocccus aureus) and gram-negative bacteria (Escherichia coli). This study used a completely randomized design (CRD) consisting of 5 treatment groups and 2 control groups with 4 repetitions. The treatment group consisted of Calotropis gigantea methanol extract with a concentration of 10%, 25%, 50%, 75% and 100% and the control group consisted of negative control using 1% Sodium Carboxy Methyl Cellulose (CMC) and positive control using ampicillin for S. aureus and chloramphenicol for E. coli bacteria. Antibacterial activity test was carried out by disc diffusion method. Phytochemical test results showed that the methanol extract of Calotropis gigantea flower contained alkaloids, steroids, terpenoids, flavonoids, saponins, tannins and coumarin. Antibacterial test results of methanol extract of Calotropis gigantea flower on Staphylocccus aureus and Escherichia coli showed that the methanol extract of Calotropis gigantea flowers had activity against Staphylocccus aureus and Escherichia coli bacteria and increased concentration of methanol extract of Calotropis gigantea flower followed by increased inhibition zone. Statistical analysis with ANOVA and Duncan test at 95% Confidence Interval showed significant differences between treatment groups.

Paper Citation

in Harvard Style

Hasballah K. (2018). Antibacterial Activity of Methanol Extract of Calotropis gigantea Flowers from Aceh.In Proceedings of the 2nd Syiah Kuala International Conference on Medicine and Health Sciences - Volume 1: SKIC-MHS, ISBN 978-989-758-438-1, pages 196-200. DOI: 10.5220/0008788801960200

in Bibtex Style

@conference{skic-mhs18,
author={Kartini Hasballah},
title={Antibacterial Activity of Methanol Extract of Calotropis gigantea Flowers from Aceh},
booktitle={Proceedings of the 2nd Syiah Kuala International Conference on Medicine and Health Sciences - Volume 1: SKIC-MHS,},
year={2018},
pages={196-200},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008788801960200},
isbn={978-989-758-438-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd Syiah Kuala International Conference on Medicine and Health Sciences - Volume 1: SKIC-MHS,
TI - Antibacterial Activity of Methanol Extract of Calotropis gigantea Flowers from Aceh
SN - 978-989-758-438-1
AU - Hasballah K.
PY - 2018
SP - 196
EP - 200
DO - 10.5220/0008788801960200