Bcl2 Gene Expression Profile on Administration of Novel Active Compound from Soursop Leaves (SF-1603) as a New Molecular Target in Liver Cancer Therapy

Maya Tejasari, Siti Aminah Abdurachman, Dwi Prasetyo, Herri S. Sastramihardja

2018

Abstract

Most cases of liver cancer present in advanced stages so the prognosis remains poor. Apoptotic dysregulation of liver cancer cells by BCL-2 gene expression is linked to tumor progression and resistance to treatment. Soursop plant compound is believed will be able to induce apoptosis by interfering Bcl2 gene expression. The objectives of the study was to explore the role of novel active compound isolated from soursop leaves againts Bcl2 gene expression in order to find new molecular target for liver cancer therapy. This study was in vitro experimental to assess active compound (SF-1603) effects on Bcl2 mRNA expression. Treatment groups were treated with SF-1603 dosage of 0,5xIC50, IC50 and 2xIC50.  Observations were assessed in hours 0, 24, 48, and 72. The results showed that Bcl-2 gene optimum expressions were achieved with 2xIC50 dose at hour 24.  There were strong correlation between Bcl-2 gene expression with apoptosis level (r=0,558). This evidence indicates that  administration of SF-1603  promote expression of Bcl-2 gene to produce a peak signal that activate the apoptosis.  It was concluded that SF-1603 affect Bcl2 gene expression as liver cancer therapy molecular target on HepG2 cell line culture.

Paper Citation

in Harvard Style

Tejasari M., Abdurachman S., Prasetyo D. and Sastramihardja H. (2018). Bcl2 Gene Expression Profile on Administration of Novel Active Compound from Soursop Leaves (SF-1603) as a New Molecular Target in Liver Cancer Therapy.In Proceedings of the 2nd Syiah Kuala International Conference on Medicine and Health Sciences - Volume 1: SKIC-MHS, ISBN 978-989-758-438-1, pages 57-64. DOI: 10.5220/0008790800570064

in Bibtex Style

@conference{skic-mhs18,
author={Maya Tejasari and Siti Aminah Abdurachman and Dwi Prasetyo and Herri S. Sastramihardja},
title={Bcl2 Gene Expression Profile on Administration of Novel Active Compound from Soursop Leaves (SF-1603) as a New Molecular Target in Liver Cancer Therapy},
booktitle={Proceedings of the 2nd Syiah Kuala International Conference on Medicine and Health Sciences - Volume 1: SKIC-MHS,},
year={2018},
pages={57-64},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008790800570064},
isbn={978-989-758-438-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd Syiah Kuala International Conference on Medicine and Health Sciences - Volume 1: SKIC-MHS,
TI - Bcl2 Gene Expression Profile on Administration of Novel Active Compound from Soursop Leaves (SF-1603) as a New Molecular Target in Liver Cancer Therapy
SN - 978-989-758-438-1
AU - Tejasari M.
AU - Abdurachman S.
AU - Prasetyo D.
AU - Sastramihardja H.
PY - 2018
SP - 57
EP - 64
DO - 10.5220/0008790800570064