2 MATERIALS AND METHODS
A field trial was set up in the Immature Plants Palm
Oil Plantation, PT. FajarAgung Lestari North
Sumatera, PerbaunganSerdangBedagai. The study
was conducted on 6 April to 10 June 2017.
Experimental plots with each size of 3 x 3 m
2
were
laid in a randomized complete block design with 3
replications. The summed dominance ratio ofA.
gangetica and Ottochloanodosa were 32 and 37%,
respectively. The other weeds were
Puerariajavanica(15%) and mixed of Cynodonsp,
Colocasiasp, Centrocemasp (14%).
The treatments were Glyphosate-isopropyl
ammonium 1.5 l/ha + Metsulfuron-methyl 150 g/ha;
Paraquat dichloride 2 l/ha; 2,4-D Dimethyl amine
2.5l/ha and untreated control (water). Spray volume
was 450 l/ha using conventional Solo Knapsack
Sprayer with red flat-fan nozzle of 5/64 inch orifice
diameter, 1-1.5 bars pressure. Assessments were
done on percent yellowing of treated weeds at 3 days
after treatment (DAT) and percentage of weeds
killed was carried out at 7, 14, 21, 28 DAT. Re-
growth of weed was evaluated at 30, 44 and 58
DAT. Wet and dry weight of weed were also
evaluated at 58 DAT (from square quadrate of 0.5
x0.5m).
One-way ANOVA was done to determine the
effect of treatments on the weed. If ANOVA results
were significant, LSD Test (P = 0.05) were used to
separate the means. Data of weed yellowing, weed
control and re-growth was normalized
5.0x
(Gomez & Gomez, 1984) before analysis. All
statistical analyses were run on the MINITAB
Statistical Package (Minitab vol.16, 2010).
3 RESULTS AND DISCUSSIONS
Herbicides affected weed yellowing. Both A.
gangetica and O. nodosa showed high weed
yellowing (up to ±80%) caused by Paraquat
dichloride 2 l/ha, whilst 2,4-D Dimethyl amine
2.5l/ha caused of 57.33 and 76.66%, respectively.
Application of Glyphosate-isopropyl ammonium 1.5
l/ha + Metsulfuron-methyl 150 g/ha caused lowest
A. gangetica and O. nodosa yellowing (23.33 and
43.33%) (Table 1).
The high effect of Paraquat dichloride 2 l / ha on
A. gangetica and O. nodosa showed yellowing of
leaves, and some of them were brown, even black ,
especially leaves at weed canopy. However, the
leaves, stems and branches on the bottom position
were still alive. Paraquat dichloride is a contact
herbicide acting by inhibition of respiration and
photosynthesis, destroying the plants foliar system
(Sebayang, 2005).
Application of 2,4-D Dimethyl amine 2.5 l / ha
caused 100% of A. gangeticakilled at 21 DAT. It
might causea selective herbicides, poisonous
especially to broadleaf weeds, mainly absorbed by
leaf and also through the soil. Whilst, Glyphosate-
isopropyl ammonium 1.5 l/ha + metsulfuron-methyl
150 g/ha caused lowest A. gangeticakilled at 7 DAT,
and it increased consistently up to 100% at 28 DAT.
Glyphosate is a systemic herbicide and it is much
more effective against weed with translocated
readily through the plant. Paraquat dichloride
generally seems to be similar effective with 2,4-D
Dimethyl amine 2.5 l/ha in controlling A. gangetica.
Paraquat dichloride 2l/ha caused high effect onO.
nodosakilled (up to 80%) at 7 DAT, whilst
Glyphosate-isopropyl ammonium 1.5 l/ha +
metsulfuron-methyl 150 g/ha and 2,4-D Dimethyl
amine 2.5 l/ha only caused weed killed 13.33 and
33.33%, respectively. Application of 2,4-D
Dimethyl amine showed weed killed ranging 30 to
40% until 28 DAT, but Glyphosate-isopropyl
ammonium 1.5 l/ha + metsulfuron-methyl 150 g/ha
showed weed killed until 28 DAT and it caused
68.33% of weed killed. Generally, herbicides of 2,4-
D Dimethyl amine 2 l/ha showed less effective in
controlling O. nodosa (Table 1). According to
Nasution (1986
a
) 2.4-D compounds included in
selective herbicides, are toxic especially to broadleaf
plants. The selectivity properties of 2,4-D herbicides
occur on the basis of recovery in broad-leaved plants
greater than that in tanning plants due to interception
and higher spray solution retention.
Paraquatdichloride 2 l/ha showed high effect at
early evaluation, but it decreased the effect at 14, 21,
and 28 DAT. Control of O. nodosa with a paraquat
herbicide did not show effective results because the
stolen part of weeds was not exposed to herbicides.
Consequently, new leaves continued to grow in the
next period. According to Wibawa et al. (2009),
some annual grasses may only be temporarily
suppressed by paraquaat, because the low and
enclosed growing points are not contacted by the
spray.
A 38.33% A. gangetica re-growth was observed
in the Paraquat dichloride 2 l/ha application.
Observation at 58 DAT also indicated the faster re-
growth duration than other application due to their
relatively higher dry weights at 58 DAT (Table 2).
Application 2,4-D Dimethyl amine on A.
gangetica(Table 2) showed the lowest weed re-