Effect of Beluntas (Pluchea indica (L.) Less) Leaves Ethanol Extract of Incision Wound and Healing in Mice (Mus musculus L.)

Emita Sabri, Rahma Handayani

2019

Abstract

A wound can be caused by punctures, collisions, bites or scratches of sharp object that can be avoid by mecanism of wound healing. The use of herbal medicine is an alternative choice to wound healing because of the relatively small side effects, one of which is Pluchea indica (L.) Less leaves ethanol extract because it contains saponin, tannin, and terpenoid as anti-inflammatory and antibacterial to accelerate the process of wound healing. The aims of this research were to find out the effectiveness of ethanol extract of beluntas leaf for duration and histological appearance process of wound healing on mice’s skin. This research used 25 male mice that were divided into five different treatment groups. The treatment groups were treated with K-, K+ (povidone-iodine) and beluntas leaf ethanol extract with three concentration of 25%, 50% and 75% ointments. Incision wound was made into 1 cm length, the ointments were applied onto the wound and observed for twice a day in 14 days. Histological preparation was made to calculate epithelial thickness, lymphocytes and fibroblasts. The data were analyzed statistically using SPSS. The result of this research showed beluntas leaves ethanol extract 25% was a faster effect on the duration of wound healing which was 6.8 days. Histological observations showed that beluntas leaf ethanol extract ointment concentration of 25% had the most significant influence of the average epithelial thickness, while concentration of 75% had the most significant influence of average number of lymphocytes and fibroblast. In conclusion, the beluntas leaves extract ointment has a positive effect on the wound healing process.

Paper Citation

in Harvard Style

Sabri E. and Handayani R. (2019). Effect of Beluntas (Pluchea indica (L.) Less) Leaves Ethanol Extract of Incision Wound and Healing in Mice (Mus musculus L.). In Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath, ISBN 978-989-758-556-2, pages 171-177. DOI: 10.5220/0010138200002775

in Bibtex Style

@conference{imc-scimath19,
author={Emita Sabri and Rahma Handayani},
title={Effect of Beluntas (Pluchea indica (L.) Less) Leaves Ethanol Extract of Incision Wound and Healing in Mice (Mus musculus L.)},
booktitle={Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,},
year={2019},
pages={171-177},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010138200002775},
isbn={978-989-758-556-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,
TI - Effect of Beluntas (Pluchea indica (L.) Less) Leaves Ethanol Extract of Incision Wound and Healing in Mice (Mus musculus L.)
SN - 978-989-758-556-2
AU - Sabri E.
AU - Handayani R.
PY - 2019
SP - 171
EP - 177
DO - 10.5220/0010138200002775