Screening Endophytic Fungi of Melaleuca cajuputi Powell Leaves as Antibacterial Sources

Hary Widjajanti, Salni, Niken Irfa Nastiti, Elisa Nurnawati

2019

Abstract

Melaleuca cajuputi leaves contain high flavonoids, flavonoids are chemical compounds that are antibacterial. Antibacterial bioactive compounds are found in plants, but the use of plants as an antibacterial source requires a large amount of biomass. Endophytic fungi that live in plant tissue can produce secondary metabolites that potentially as an antibacterial compounds. Isolation of the endophytic fungi for antibacterial sources can reduce the large amount of plant as a source of antibacterial agents. The aims of this research were obtain endophytic fungi of M.cajuputi leaves as an antibacterial sources. The research stages were sampling, isolation and purification of endophytic fungi, cultivation and production of secondary metabolites, antibacterial activity and the minimum inhibitory concentration (MIC) tests, thin layer chromatography, and characterization and identification of endophytic fungi that potentially as an antibacterial sources. The results there were 7 isolates of endophytic fungi from Melaleuca cajuputi leaves. MC1, MC2, MC3, and MC4 fungal isolates have a strong antibacterial activities. The MIC of MC2 and MC3 extracts on Escherichia coli were 400 μg/mL and 100 μg/mL. The MIC of MC1 and MC4 extracts on Staphylococcus aureus were 200 μg/mL and 700 μg/mL, respectively. The extract of four endophytic fungi contain phenol and flavonoid that potentially as an antibacterial. Endophytic fungi MC1 isolate identified as Scopulariopsis candida, MC2 isolate as Fusarium equiseti, MC3 isolate as Fusarium sporotrichoides, and MC4 isolate as Mucor hiemalis.

Paper Citation

in Harvard Style

Widjajanti H., Salni., Irfa Nastiti N. and Nurnawati E. (2019). Screening Endophytic Fungi of Melaleuca cajuputi Powell Leaves as Antibacterial Sources. In Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath, ISBN 978-989-758-556-2, pages 196-204. DOI: 10.5220/0010139000002775

in Bibtex Style

@conference{imc-scimath19,
author={Hary Widjajanti and Salni and Niken Irfa Nastiti and Elisa Nurnawati},
title={Screening Endophytic Fungi of Melaleuca cajuputi Powell Leaves as Antibacterial Sources},
booktitle={Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,},
year={2019},
pages={196-204},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010139000002775},
isbn={978-989-758-556-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,
TI - Screening Endophytic Fungi of Melaleuca cajuputi Powell Leaves as Antibacterial Sources
SN - 978-989-758-556-2
AU - Widjajanti H.
AU - Salni.
AU - Irfa Nastiti N.
AU - Nurnawati E.
PY - 2019
SP - 196
EP - 204
DO - 10.5220/0010139000002775