Antioxidant Activity of Ethanol Extract of Okra (Abelmoschus esculentus (L.) Moench) and Its Effect on the Expression of P53 in Breast Cancer Rat Model

Mhd Al Amin Nasution, Syarifah Riska Mela Putri, Salomo Hutahaean, Syafruddin Ilyas, Widya Sahfitri, Fitri Elizabeth

2019

Abstract

Breast cancer is a type of cancer with a high incidence in Indonesia, ranking second highest after cervical cancer. The search for effective therapeutic agents for breast cancer, including the investigations of natural agents, still needs to be done. The study was conducted to evaluate the anticancer effect of seed pods extracts of okra (Abelmoschus esculentus L., Moech) which is traditionally used in the treatment of various diseases. The objectives of the study were to determine the antioxidant activity of okra seed pods extracts and evaluate the effect of the extract on tumor severity in the benzo(α)pyrene induced rat breast cancer model. The experiment was carried out using a completely randomized design, with 5 treatments and 6 replications. These treatments were: 2 control groups (K0 = normal group; K1 = breast cancer model rat due to benzo(α)pyrene injection), and 3 extract groups (breast cancer model rats were given okra seed pods extract dose of 150, 300, and 450 mg /kg body weight, respectively). The results showed that okra fruit ethanol extract has a strong antioxidant activity with IC50 value = 68.79, but the extract has not been able to trap free radicals due to the induction of benzo(α)pyrene in test animals. Immunohistochemical examination showed that there was no significant difference and effect between ethanol extract of okra fruit and wild type p53 expression in test animals induced with benzo(α)pyrene cancer growth compounds.

Paper Citation

in Harvard Style

Nasution M., Putri S., Hutahaean S., Ilyas S., Sahfitri W. and Elizabeth F. (2019). Antioxidant Activity of Ethanol Extract of Okra (Abelmoschus esculentus (L.) Moench) and Its Effect on the Expression of P53 in Breast Cancer Rat Model. In Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath, ISBN 978-989-758-556-2, pages 509-513. DOI: 10.5220/0010208300002775

in Bibtex Style

@conference{imc-scimath19,
author={Mhd Al Amin Nasution and Syarifah Riska Mela Putri and Salomo Hutahaean and Syafruddin Ilyas and Widya Sahfitri and Fitri Elizabeth},
title={Antioxidant Activity of Ethanol Extract of Okra (Abelmoschus esculentus (L.) Moench) and Its Effect on the Expression of P53 in Breast Cancer Rat Model},
booktitle={Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,},
year={2019},
pages={509-513},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010208300002775},
isbn={978-989-758-556-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,
TI - Antioxidant Activity of Ethanol Extract of Okra (Abelmoschus esculentus (L.) Moench) and Its Effect on the Expression of P53 in Breast Cancer Rat Model
SN - 978-989-758-556-2
AU - Nasution M.
AU - Putri S.
AU - Hutahaean S.
AU - Ilyas S.
AU - Sahfitri W.
AU - Elizabeth F.
PY - 2019
SP - 509
EP - 513
DO - 10.5220/0010208300002775