The Potential Methanolic Extract of Coffee Leaves (Coffea canephora L.) in Inhibiting Storage Fungi and Yeast

Anggi Audina, Kiki Nurtjahja, Albert Pasaribu

2019

Abstract

Robusta coffee (Coffea canephora L.) leaves contain alkaloids, flavonoids, and phenolics that antifungal activity. This study aims to determine the ability of robusta coffee leaf extract in inhibiting six spesies of postharvest fungi and yeast (Aspergillus candidus, Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus oryzae, Aspergillus tamarii, Penicillium sp., Candida albicans and yeast). The leaves were obtained from farmers at Berastagi, Karo Regency, North Sumatra. The extract was obtained by maceration using methanol. Antifungal activity was determined using disc diffusion method at concentrations 20, 40, 60, 80 and 100%. The minimum inhibitory concentrations were determined by dilution method at concentrations 20, 40, 60, 80, 85, 90, and 95%.The positive control used was ketoconazole 2% and negative control dimethyl sulfoxide 10%. Results showed coffee leaves extract has anti-fungal activity againts storage fungi and yeast which were characterized by an inhibition zone from 9.27 to 0.62 mm. The most effective extract at concentration 100% with the highest inhibition 9.27 mm againts A. oryzae. Whereas, the minimum inhibitory concentration was at 85% againts A. oryzae and C. albicans.

Paper Citation

in Harvard Style

Audina A., Nurtjahja K. and Pasaribu A. (2019). The Potential Methanolic Extract of Coffee Leaves (Coffea canephora L.) in Inhibiting Storage Fungi and Yeast. In Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath, ISBN 978-989-758-556-2, pages 534-537. DOI: 10.5220/0010612500002775

in Bibtex Style

@conference{imc-scimath19,
author={Anggi Audina and Kiki Nurtjahja and Albert Pasaribu},
title={The Potential Methanolic Extract of Coffee Leaves (Coffea canephora L.) in Inhibiting Storage Fungi and Yeast},
booktitle={Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,},
year={2019},
pages={534-537},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010612500002775},
isbn={978-989-758-556-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,
TI - The Potential Methanolic Extract of Coffee Leaves (Coffea canephora L.) in Inhibiting Storage Fungi and Yeast
SN - 978-989-758-556-2
AU - Audina A.
AU - Nurtjahja K.
AU - Pasaribu A.
PY - 2019
SP - 534
EP - 537
DO - 10.5220/0010612500002775