The Effect of Basil Leaves Extract (Ocimum sanctum L.) on Mycelial Growth of Postharvest Fungi

Kiki Nurtjahja, Albert Pasaribu, Roslindawati

2019

Abstract

Basil (Ocimum sanctum L.) contain secondary metabolites which inhibit fungal grow. This research aims to investigate methanolic extract of basil leaves against postharvest fungi. Dried basil leaves were extracted and macerated using methanol. The extract was stored for 3×24 hours at room temperature and homogenized for 24 hours. The homogenise was filtered using whatman filter paper and evaporated at 50 ºC. The thick extract was made a serial concentration 100, 80, 60, 40 and 20 % by dimethylsulfoxide 10%. The postharvest fungi used were Aspergillus oryzae, Aspergillus flavus, Aspergillus fumigatus, Aspergillus tamarii, Aspergillus niger, Aspergillus candidus, and Penicillium sp. The minimum inhibitory concentration was determined by liquid dilution. Ketoconazole 2% and dimethyl sulfoxide were used as positive and negative control respectively. Results showed that basil leaves with concentration 100, 80, 60, 40 and 20% inhibit fungal growth, the highest inhibition occurred on Aspergillus tamarii with inhibition 8.7; 8.0; 7.2; 6.0; 5.0 mm respectively. The lowest inhibition occurred on Penicillium sp. with inhibition 2.5; 2.0, 1.8; 0.0 mm respectively.

Paper Citation

in Harvard Style

Nurtjahja K., Pasaribu A. and Roslindawati. (2019). The Effect of Basil Leaves Extract (Ocimum sanctum L.) on Mycelial Growth of Postharvest Fungi. In Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath, ISBN 978-989-758-556-2, pages 542-544. DOI: 10.5220/0010612700002775

in Bibtex Style

@conference{imc-scimath19,
author={Kiki Nurtjahja and Albert Pasaribu and Roslindawati},
title={The Effect of Basil Leaves Extract (Ocimum sanctum L.) on Mycelial Growth of Postharvest Fungi},
booktitle={Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,},
year={2019},
pages={542-544},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010612700002775},
isbn={978-989-758-556-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,
TI - The Effect of Basil Leaves Extract (Ocimum sanctum L.) on Mycelial Growth of Postharvest Fungi
SN - 978-989-758-556-2
AU - Nurtjahja K.
AU - Pasaribu A.
AU - Roslindawati.
PY - 2019
SP - 542
EP - 544
DO - 10.5220/0010612700002775