The Potential of Proteolitic Bacteria Isolated from Peat Soil against Salmonella typhi and Escherichia coli

Rizni Syahputri, Rachmiati, Sartini, Kiki Nurtjahja

2019

Abstract

Peat soil consisted of organic organic soil that developed of plant residue in wet condition. The aim of this study was to investigate the ability of protelytic bacteria originated from peat soil in inhibiting pathogenic bacteria (Salmonella typhi and Escherichia coli). The soil samples were obtained from an area ±200 m2 at Labuhan Batu, North Sumatera, Indonesia. Each soil samples (200 g) were homogenized and serial dilution was conducted to isolate soil bacteria. All of the bacteria then were cultured in skim milk agar at 37°C for 48 h. Bacterial colony with clear zone were isolated and identified. Nutrient agar plate medium was used to examine antagonist characteristics between pathogenic and proteolytic bacteria. Results showed that total 12 isolates bacteria were succesfully isolated. Among 12 isokates, six isolates (PSp7, PSp8, PSp9, PSp10, PSp11 dan PSp12) were proteolytic characteristics. Among the proteolytic isolates, PSp7b and PSp11 were the highest clear zone. However, antagonism of isolate PSp10 (14.88 mm) againts E. coli was higher than that of PSp9 (10.25 mm) againts S. typhi.

Paper Citation

in Harvard Style

Syahputri R., Rachmiati., Sartini. and Nurtjahja K. (2019). The Potential of Proteolitic Bacteria Isolated from Peat Soil against Salmonella typhi and Escherichia coli. In Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath, ISBN 978-989-758-556-2, pages 545-547. DOI: 10.5220/0010612900002775

in Bibtex Style

@conference{imc-scimath19,
author={Rizni Syahputri and Rachmiati and Sartini and Kiki Nurtjahja},
title={The Potential of Proteolitic Bacteria Isolated from Peat Soil against Salmonella typhi and Escherichia coli},
booktitle={Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,},
year={2019},
pages={545-547},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010612900002775},
isbn={978-989-758-556-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,
TI - The Potential of Proteolitic Bacteria Isolated from Peat Soil against Salmonella typhi and Escherichia coli
SN - 978-989-758-556-2
AU - Syahputri R.
AU - Rachmiati.
AU - Sartini.
AU - Nurtjahja K.
PY - 2019
SP - 545
EP - 547
DO - 10.5220/0010612900002775