Fungal Contamination and Toxigenic Aspergillus flavus Strains on Dried Anchovies Sold in North Sumatera

Jesica Simanullang, Kiki Nurtjahja

2019

Abstract

Dried anchovies at Medan traditional markets commonly sold unpacked and stored in open air, as the result, the dried-salted fish was easily spoiled and contaminated by mycotoxigenic fungi. The aims of the study were to enumerate fungal population and to characterize A. flavus strains. Each fungal species were isolated from medan anchovies at five traditional markets. The percentage of fungal contamination was determined by a direct plating using dichloran 18% glycerol agar medium. Fungal population was determined using a serial dilutions and pour plate in DG18 medium. Toxigenic Aspergillus flavus strains were characterized by the production of yellow pigment on the reverse side of petri dishes containing coconut agar medium 10%. The presence of regulatory (aflR) and structural genes (nor-1, ver-1 and omt-1) genes determined aflatoxin biosynthesis was determined by amplifying four sets of specific primers (aflR, nor-1, ver-1 and omt-1). Results showed that medan anchovies were contaminated by storage fungi. Aspergillus niger was the most common found followed by A. flavus and A. chevalieri. Eighty strains of A. flavus were isolated from all samples, they are consisted 69 toxigenic and 11 strains were non-toxigenic.

Paper Citation

in Harvard Style

Simanullang J. and Nurtjahja K. (2019). Fungal Contamination and Toxigenic Aspergillus flavus Strains on Dried Anchovies Sold in North Sumatera. In Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath, ISBN 978-989-758-556-2, pages 548-551. DOI: 10.5220/0010613000002775

in Bibtex Style

@conference{imc-scimath19,
author={Jesica Simanullang and Kiki Nurtjahja},
title={Fungal Contamination and Toxigenic Aspergillus flavus Strains on Dried Anchovies Sold in North Sumatera},
booktitle={Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,},
year={2019},
pages={548-551},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010613000002775},
isbn={978-989-758-556-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,
TI - Fungal Contamination and Toxigenic Aspergillus flavus Strains on Dried Anchovies Sold in North Sumatera
SN - 978-989-758-556-2
AU - Simanullang J.
AU - Nurtjahja K.
PY - 2019
SP - 548
EP - 551
DO - 10.5220/0010613000002775