Antibacterial Activity of Watercress (Nasturtium Officinale) against Staphylococcus Aureus

Abdul Karim, Yuni Sartika, Kiki Nurtjahja

2019

Abstract

The aims of the study was to investigate the potential of leaf ethanol extract of watercress (Nasturtium officinale) againts Staphyllococcus aureus. The air-dried of watercress were ground and extracted using ethanol 70% and stored for 3 days. The macerate then was filtered and evaporated at 50°C until thick paste ectract was obtained. Extract was made several concentration by dimethyl sulfoxide 10%. The concentration used was 0, 60, 75, and 100%. As much as 0.1 ml bacterial suspension (108 colony forming unit/ml) was cultured in 15 ml mueller hinton agar (MHA) in petri dish (9 cm in diameter) and paper disc (0.5 mm in diameter) was soaked by extract and placed on the surface of the MHA medium. Blank paper disc soaked with distilled water and chloramphenicol as negative and positive control respectively. Three replicates for each treatment. All plates were incubated at 37°C for 24 h. The presence of clear zone surrounding paper disc was observed. The minimum inhibitory concentration (MIC) was determined based on the clear zone on each extract concentration. Results showed that inhibition occured at extract concentration 100% (4.5 mm) and MIC at 90% (2.42 mm).

Paper Citation

in Harvard Style

Karim A., Sartika Y. and Nurtjahja K. (2019). Antibacterial Activity of Watercress (Nasturtium Officinale) against Staphylococcus Aureus. In Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath, ISBN 978-989-758-556-2, pages 552-554. DOI: 10.5220/0010613100002775

in Bibtex Style

@conference{imc-scimath19,
author={Abdul Karim and Yuni Sartika and Kiki Nurtjahja},
title={Antibacterial Activity of Watercress (Nasturtium Officinale) against Staphylococcus Aureus},
booktitle={Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,},
year={2019},
pages={552-554},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010613100002775},
isbn={978-989-758-556-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,
TI - Antibacterial Activity of Watercress (Nasturtium Officinale) against Staphylococcus Aureus
SN - 978-989-758-556-2
AU - Karim A.
AU - Sartika Y.
AU - Nurtjahja K.
PY - 2019
SP - 552
EP - 554
DO - 10.5220/0010613100002775