Based on the plotted graph (data retrieval was
started from the drug released in the colon because
the drug began to be absorbed at that time), a
correlation value was obtained (R
2
= 0.8785). It can
be stated that there is a correlation between the
formulations tested in vitro and in vivo because the
correlation value was greater than 0.8, it is assumed
to have a correlation (Dalimunthe et al. 2019,
Shargel 1988).
The in vivo test was carried out for 15 hours, but
the new drug was released at 7 hours, which was
about 0.1888 µg / ml, this means that it can be
proven that the capsule is not destroyed in the in
vivo test either in the stomach or in the intestine, but
the intestines have begun to expand, and you can see
that the drug begins to release and there is an
increase in the percentage of drug release at 8 hours.
Based on the reaction kinetics, the in vitro test
(dissolution) shows that the drug follows order 1,
order zero, and higuchi but it tended to be order zero
because the release is relatively constant.
4 CONCLUSION
Corncob hemicellulose capsule considered as
altenate carrier for colon-targeted drug, it was
recorded that the drug release was occurred at pH 8
and 7 hours post oral administration. Therefore, it
has distinctive properties with conventional gelatin
capsule which is vulnerable to stomach acidic
conditions. In vitro in vitro correlation (IVIVC)
study also exhibites a strong relationship which was
indicated by the value of R
2
= 0.8785. It
demonstrated that in vitro dissolution test of
metronidazole microparticle in corncob
hemicellulose capsule is of high relevance for in
vivo assay.
REFERENCES
Ahuja, S., Dong, M., W, Eds. 2005. Handbook of
Pharmaceutical Analysis HPLC edisi 1 United
Kingdom: Elsevier, Inc.,.p. 191-217, 401- 412.
Amidon, S., Brown, J.E., Dave, VS. 2015. Colon-targeted
oral drug delivery systems: Design Trends and
approaches. American Association of Pharmaceutical
Scientist Pharm. Sci.Tech. 16(4) 731-741.
Chilukuri, D.M., Suskara G., Young, D. 2007.
Pharmaceutical Product Development in vitro - in vivo
Corelation, Informa USA, New York. (165):110-
165.
Chow, Shein-Chung, Liu-Pei. 2003. Design and Analysis
of Clinical Trials: Concepts and Methodologies, 3
rd
Edition. Wiley.
Cliceri, D., Spinelli, S., Dinella, C., Prescott, J.,
Monteleone, E. 2018. The influence of psychological
traits, beliefs and taste redponsiveness on implicit
attitudes toward plant-and animal-based dishes among
vegetarians, flexitarians and omnivores. Food Quality
and Preference. Vol 68. 276-291.
Dalimunthe, G.I., Muchlisyam, Urip Harahap, Azizah
Nasution. 2020. The Effect of Variation In The
Amount of Hemicellulose from Corncob (Zea mays L)
and pH On Metronidazole Microparticle Release. IOP
Conf. Series: Journal Of Physics:
Conf.Series.1462.01203.
Dalimunthe, G.I., Muchlisyam Bachri, U. Harahap, A.
Nasution. 2019. Formulation of capsules shell from
corncob hemicellulose combined with isolated sodium
alginate. rasayan Journal of Chemistry: ISSN: 0974-
1496 Rasayan J Chem. Vol. 12(3): 1668-1675.
Emami, J. 2006. In vitro- in vivo correlation: From the
theory to applications. Journal of Pharmacy and
Pharmaceutical Sciences. 9(2): 169-189.
Kemenkes RI. 2014. Farmakope Indonesia. Edisi V.
Jakarta.Departemen Kesehatan RI
Lee, S.H., Bajracharya, R., Min. J.Y., Han, J.W., Park,
B.J., han, H.K. 2020. Strategic approaches for colon
targeted drug delivery: an overview of recent
advancements. Journal of Pharmaceutics. 12(1) 68.
Maity, S., Kundu A., Karmakar S., Biswanath, 2016, In
Vitro and In vivo Correlation of Colon-Targeted
Compression-Coated Tablets, Hindawi Publishing
Corporation. Journal of Pharmaceutics. vol. 2016,
Article.
Muchlisyam, 2014. Corn Cobs Hemicelluloses Isolation
Method Comparison and its Characterization with
Infra Red Spectrophotometry (FTIR) and High
Perfomance Liquid Chrromatography (HPLC).
International Journal of Chem Tech Research CODEN
(USA): IJCRGG ISSN: 0974-4290 vol.6 No.5.pp
3062-3070.
Muchlisyam, Sumaiyah, 2016. Application of corn cobs
hemiselulose microparticle, carrier with insulin as
model. Der Pharma Chemica 8(19): 632: 641.
Muhaimin. 2013, Study of Microparticle Preparation by
The Solvent Evaporation Method Using Focused
Beam Reflectance Measurement (FBRM) Disertation
Nicholas, E.M., Panaganti, S., Prabakaran, l., Jayveera,
KN. 2011. Novel colon spesific drug delivery system:
a review. International Journal of Pharmaceutical
Sciences and Research. Vol 2(10): 2545-2561.
Oladzadabbasabadi, N., Ebadi, S., Nafchi, A.M., Karim,
A.A., Kiahosseini, SR. 2017. Functional properties of
dually/K-carrageenan films: an alternative to gelatin in
pharmaceutical capsules. Carbohydrate Polymers. Vol.
160. 43-51.
Qiu, Y., Duan J.Z. 2017. Chapter 16-In vitro/ in vivo
correlations: Fundamentals, development
considerations and applications. Developing Solid