Extraction and Analysis of Nicotine from the Saliva of Active Smokers using UV Spectroscopy

Muhammad Taufik, Desi Ardilla, Mariany Razali, Endang Susilawati, Afniwati, Nurul Fadillah

2019

Abstract

An accurate and simple method of extraction and analysis needs to be developed in the context of investigating nicotine in the saliva of active smokers. This study aims to prepare, extract and analyse nicotine from the saliva of active smokers. The preparation process is carried out from the location where the samples were taken. Extraction was carried out using sonication coupling maceration for 15 minutes. Qualitative analysis used spot test with Cyanogen bromide reagent and quantitative analysis used UV spectrophotometer. Ultra Violet (UV) spectrophotometer analysis at the optimum wavelength of 260 nm resulted in a sample concentration of Saliva A = 1.4 ppm and Saliva B = 1.6 ppm

Paper Citation

in Harvard Style

Taufik M., Ardilla D., Razali M., Susilawati E., Afniwati. and Fadillah N. (2019). Extraction and Analysis of Nicotine from the Saliva of Active Smokers using UV Spectroscopy. In Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath, ISBN 978-989-758-556-2, pages 616-620. DOI: 10.5220/0010614700002775

in Bibtex Style

@conference{imc-scimath19,
author={Muhammad Taufik and Desi Ardilla and Mariany Razali and Endang Susilawati and Afniwati and Nurul Fadillah},
title={Extraction and Analysis of Nicotine from the Saliva of Active Smokers using UV Spectroscopy},
booktitle={Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,},
year={2019},
pages={616-620},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010614700002775},
isbn={978-989-758-556-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,
TI - Extraction and Analysis of Nicotine from the Saliva of Active Smokers using UV Spectroscopy
SN - 978-989-758-556-2
AU - Taufik M.
AU - Ardilla D.
AU - Razali M.
AU - Susilawati E.
AU - Afniwati.
AU - Fadillah N.
PY - 2019
SP - 616
EP - 620
DO - 10.5220/0010614700002775