Genetic Diversity of Aspergillus Flavus Isolated from Pepper at North Sumatera

Kiki Nurtjahja, Cut Fatimah Zuhra, Helmina Sembiring, Betriana N. L. Gultom

2019

Abstract

Aspergillus flavus is one of the postharvest fungi that often infects white and black pepper (Piper nigrum L.). Fungal infection on pepper not only spoiled physically but also produce mycotoxins. The aim was to study Aspergillus flavus toxigenicity and their genetic diversity on dried-stored white and black pepper sold at traditional markets in North Sumatera. Fungal population was determined based on colony forming unit per mililiter (cfu/mL) using serial dilutions and pour plated in dichloran 18% glycerol agar (DG18) medium. Toxigenicity of A. flavus was determined culturally using coconut agar medium (CAM) 10%. Phylogenetic between A. flavus strains were carried out by isolating genome of representative toxigenic and non-toxigenic Aspergillus flavus strains according to the MiniKit Promega protocol and amplified using 10 Random Amplified Polymorphic DNA (RAPD) primers. The amplified bands were scored and translated to be biner data, then analyzed using Numerical Taxonomy and Multivariate System (NTSys) and clustered by Unweighted Pair Group Method with Arithmatic Average Algorithm (UPGMA). Results showed that a total of thirty one A. flavus strains were isolated. Based on toxigenicity determination found that twelve strains of A. flavus were toxigenic (aflatoxin producers) and nineteen strains were non-toxigenic (non-aflatoxin producers). Dendogram of similar bands was constructed and showed the highest similarity coefficient of A. flavus strains was 0.84 (84%) which means that the strains were similar even though they were isolated from different traditional markets.

Paper Citation

in Harvard Style

Nurtjahja K., Zuhra C., Sembiring H. and Gultom B. (2019). Genetic Diversity of Aspergillus Flavus Isolated from Pepper at North Sumatera. In Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath, ISBN 978-989-758-556-2, pages 631-635. DOI: 10.5220/0010766800002775

in Bibtex Style

@conference{imc-scimath19,
author={Kiki Nurtjahja and Cut Fatimah Zuhra and Helmina Sembiring and Betriana N. L. Gultom},
title={Genetic Diversity of Aspergillus Flavus Isolated from Pepper at North Sumatera},
booktitle={Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,},
year={2019},
pages={631-635},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010766800002775},
isbn={978-989-758-556-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International MIPAnet Conference on Science and Mathematics - Volume 1: IMC-SciMath,
TI - Genetic Diversity of Aspergillus Flavus Isolated from Pepper at North Sumatera
SN - 978-989-758-556-2
AU - Nurtjahja K.
AU - Zuhra C.
AU - Sembiring H.
AU - Gultom B.
PY - 2019
SP - 631
EP - 635
DO - 10.5220/0010766800002775