Identification for a Large-volume Food-borne Bacteria on a Fully Integrated Portable Centrifugal Disc

Hau Van Nguyen, Van Dan Nguyen, Eun Yeol Lee, Tae Seok Seo

2019

Abstract

Herein, we present a fully integrated portable centrifugal microsystem for multiplex detection of food poisoning bacteria with a large volume of sample up to 1 mL. The microsystem consists of a portable rotary genetic analyzer and a fully integrated lab-on-a-disc device. The portable rotary genetic analyzer is equipped with a couple of heating blocks, a motor and a UV-Vis optical detector. The device was designed with two units: a 3D printed solution-loading cartridge and a centrifugal microfluidic disc. All the essential solutions for the LAMP reaction (a sample, a washing, an elution and a LAMP cocktail solution) are stored inside the cartridge, and orderly released into centrifugal microdevice by a rotation program. Each unit of the device was designed with 20 reaction chambers for simultaneously detecting 19 kinds of food poisoning bacteria in one test. To increase the amount of a sample to 1 mL, we incorporated the super absorbent polymer (SAP) in the waste chamber to absorb the sample and washing solution during the device operation. The whole process was automatically conducted from bead-based DNA extraction to isothermal DNA amplification by EBT-mediated LAMP reaction to colorimetric and UV-vis detection of amplicons in 60 min to identify three kinds of bacteria (Escherichia coli O157:H7, Salmonella Typhimurium, and Vibrio parahaemolyticus).

Paper Citation

in Harvard Style

Van Nguyen H., Nguyen V., Lee E. and Seo T. (2019). Identification for a Large-volume Food-borne Bacteria on a Fully Integrated Portable Centrifugal Disc. In Proceedings of the 12th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2019) - Volume 1: BIODEVICES; ISBN 978-989-758-353-7, SciTePress, pages 114-118. DOI: 10.5220/0007690401140118

in Bibtex Style

@conference{biodevices19,
author={Hau Van Nguyen and Van Dan Nguyen and Eun Yeol Lee and Tae Seok Seo},
title={Identification for a Large-volume Food-borne Bacteria on a Fully Integrated Portable Centrifugal Disc},
booktitle={Proceedings of the 12th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2019) - Volume 1: BIODEVICES},
year={2019},
pages={114-118},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007690401140118},
isbn={978-989-758-353-7},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 12th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2019) - Volume 1: BIODEVICES
TI - Identification for a Large-volume Food-borne Bacteria on a Fully Integrated Portable Centrifugal Disc
SN - 978-989-758-353-7
AU - Van Nguyen H.
AU - Nguyen V.
AU - Lee E.
AU - Seo T.
PY - 2019
SP - 114
EP - 118
DO - 10.5220/0007690401140118
PB - SciTePress