Phytochemical Screening and Antimicrobial Activity of Limonia acidissima Ethanol Extract Against Microbes from Clinical Isolates

Ahmad Shobrun Jamil, Siti Rofida, Dian Priyani, Wahidatin Nabila, Evi Wulandari

2019

Abstract

The aim of this study is to determine the content of secondary metabolites ethanol fraction of fruit peel of Limonia acidissima qualitatively and test the activity of antimicrobial on Escherichia coli, Staphylococcus aureus, and Candida albicans. Phytochemical screening was carried out by running for separating the extract in silica gel by eluent n-hexane: ethyl acetate. The Thin Layer Chromatography (TLC) plate results then sprayed with a stain viewer; Alkaloid stain viewer: Dragendorff reagent; Terpenoids: Anisaldehyde-sulfuric acid reagents. The plate is heated to a temperature of 100°C; Flavonoids: H2 SO4 10% reagents; Polyphenols and tannins: FeCl 3 reagents 10%; Anthraquinone: 10% KOH reagent in methanol. The antimicrobial test was carried out using disk diffusion methods with concentrations of 50 mg/ml, 75 mg/ml and 100 mg/ml with control of nystatin and chloramphenicol. Phytochemical screening results of ethanol fraction Limonia acidissima can be detected in the content of alkaloids, terpenoids, anthraquinones, and saponins. Antimicrobial test results obtained data that the ethanol fraction Limonia acidissima has an increasing inhibitory activity of 50 mg/ml, 75 mg/ml, and 100 mg/ml.

Paper Citation

in Harvard Style

Jamil A., Rofida S., Priyani D., Nabila W. and Wulandari E. (2019). Phytochemical Screening and Antimicrobial Activity of Limonia acidissima Ethanol Extract Against Microbes from Clinical Isolates.In Proceedings of the 2nd Health Science International Conference - Volume 1: HSIC, ISBN 978-989-758-462-6, pages 152-156. DOI: 10.5220/0009126001520156

in Bibtex Style

@conference{hsic19,
author={Ahmad Shobrun Jamil and Siti Rofida and Dian Priyani and Wahidatin Nabila and Evi Wulandari},
title={Phytochemical Screening and Antimicrobial Activity of Limonia acidissima Ethanol Extract Against Microbes from Clinical Isolates},
booktitle={Proceedings of the 2nd Health Science International Conference - Volume 1: HSIC,},
year={2019},
pages={152-156},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0009126001520156},
isbn={978-989-758-462-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd Health Science International Conference - Volume 1: HSIC,
TI - Phytochemical Screening and Antimicrobial Activity of Limonia acidissima Ethanol Extract Against Microbes from Clinical Isolates
SN - 978-989-758-462-6
AU - Jamil A.
AU - Rofida S.
AU - Priyani D.
AU - Nabila W.
AU - Wulandari E.
PY - 2019
SP - 152
EP - 156
DO - 10.5220/0009126001520156