Characterization and Prospect of Irradiated Chitosan as Nano Complex Material to Deliver MicroRNA in Cancer Therapy

Firasti Agung Nugrahening Sumadi, Dian Pribadi Perkasa, Tirta Wardana, Ronny Martien, Sofia Mubarika Harjana

2019

Abstract

Chitosan is odorless white powder derived from the partial deacetylation of chitin which is a polysaccharide consisting of glucosamine and N-acetylglucosamine. Chitosan is commercially available in several types and has molecular weights that vary between 10,000 and 1,000,000. Chitosan has positively charged basic chain that can easily form nano complex with nucleic acid in this case including negatively charged microRNA. MicroRNA (miRNA) has a large role in the regulation of cancer signaling tissue so that a therapeutic approach is needed to restore the balance of dysregulated miRNA. The nature of microRNA which is very susceptible to enzyme degradation requires a special system so that it is competent to deliver microRNA into the cytoplasm. One of the factors that influence the efficiency of transfection of chitosan nano complex with a nucleic acid to body cells is molecular weight. In this research, the chitosan molecular weight reduction method was carried out to increase nano complex delivery using gamma-ray irradiation. Furthermore, characterization was carried out to determine the irradiated chitosan molecular weight using intrinsic viscosity then proceed with FTIR analysis to determine changes in chemical structure and applied further by using it in nano complex formulations with microRNA 155-p, a microRNA that experienced downregulation in ovarian cancer thus requiring mimic therapy. Results showed a decrease in chitosan molecular weight after being irradiated from 110,188 dalton to 15,209 dalton while FTIR spectra showed a break of the 1-4 glycoside bonds which was equivalent to the severance of the main chain of polysaccharides. Electrophoresis results showed that irradiated chitosan was able to form nano complex with 155-5p microRNA but transfection was not able to deliver 155-5p microRNA into the SKOV3 ovarian cancer cells.

Paper Citation

in Harvard Style

Sumadi F., Perkasa D., Wardana T., Martien R. and Harjana S. (2019). Characterization and Prospect of Irradiated Chitosan as Nano Complex Material to Deliver MicroRNA in Cancer Therapy.In Proceedings of the 2nd Health Science International Conference - Volume 1: HSIC, ISBN 978-989-758-462-6, pages 191-196. DOI: 10.5220/0009127001910196

in Bibtex Style

@conference{hsic19,
author={Firasti Agung Nugrahening Sumadi and Dian Pribadi Perkasa and Tirta Wardana and Ronny Martien and Sofia Mubarika Harjana},
title={Characterization and Prospect of Irradiated Chitosan as Nano Complex Material to Deliver MicroRNA in Cancer Therapy},
booktitle={Proceedings of the 2nd Health Science International Conference - Volume 1: HSIC,},
year={2019},
pages={191-196},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0009127001910196},
isbn={978-989-758-462-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd Health Science International Conference - Volume 1: HSIC,
TI - Characterization and Prospect of Irradiated Chitosan as Nano Complex Material to Deliver MicroRNA in Cancer Therapy
SN - 978-989-758-462-6
AU - Sumadi F.
AU - Perkasa D.
AU - Wardana T.
AU - Martien R.
AU - Harjana S.
PY - 2019
SP - 191
EP - 196
DO - 10.5220/0009127001910196