Antioxidant Properties of Sweet Orange Peels in Several Fractions of Methanolic Extract

Suhartomi, Kristin Natalia Gulo, Albert Daniel Saragih, Ahmad Raif Martinus, Refi Ikhtiari

2019

Abstract

Citrus sinensis is a fruit that is widely consumed over the world and has potent natural antioxidant activity. This study was aimed to determine the potential of antioxidants from each partition of sweet orange peel methanol extract. The sweet orange peel methanol extract was made by the maceration method followed by fractionation through a separating funnel with n-hexane and dichloromethane solvents. Antioxidant activity of the methanolic extract was measured by H2O2 and DPPH methods. Results showed that the most potent H2O2 inhibition activity was the fraction of n-hexane (IC50: 91.33 µg/ml), followed by dichloromethane (IC50: 174 µg/ml), and methanol (IC50: 189.63 µg/ml). While the most potent DPPH inhibitory activity was the n-hexane fraction (IC50: 709.60 µg/ml), followed by the methanol fraction (IC50: 790.64 µg/ml) and the dichloromethane fraction (IC50: 895.58 µg ml). The fraction of n-hexane has higher antioxidant potential than the fraction of dichloromethane and methanol. These antioxidant properties might be related to bioactive compounds in the methanolic extracts which will be investigated further. However, the research results on several fractions might provide a preliminary study on citrus-based drug preparation in the pharmacy industry for antioxidants application.

Paper Citation

in Harvard Style

Suhartomi., Gulo K., Saragih A., Martinus A. and Ikhtiari R. (2019). Antioxidant Properties of Sweet Orange Peels in Several Fractions of Methanolic Extract.In Proceedings of the International Conference on Health Informatics and Medical Application Technology - Volume 1: ICHIMAT, ISBN 978-989-758-460-2, pages 371-378. DOI: 10.5220/0009515503710378

in Bibtex Style

@conference{ichimat19,
author={Suhartomi and Kristin Natalia Gulo and Albert Daniel Saragih and Ahmad Raif Martinus and Refi Ikhtiari},
title={Antioxidant Properties of Sweet Orange Peels in Several Fractions of Methanolic Extract},
booktitle={Proceedings of the International Conference on Health Informatics and Medical Application Technology - Volume 1: ICHIMAT,},
year={2019},
pages={371-378},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0009515503710378},
isbn={978-989-758-460-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the International Conference on Health Informatics and Medical Application Technology - Volume 1: ICHIMAT,
TI - Antioxidant Properties of Sweet Orange Peels in Several Fractions of Methanolic Extract
SN - 978-989-758-460-2
AU - Suhartomi.
AU - Gulo K.
AU - Saragih A.
AU - Martinus A.
AU - Ikhtiari R.
PY - 2019
SP - 371
EP - 378
DO - 10.5220/0009515503710378