The Differences of Electrophoretic Profile and Snake Venom Phospholipase A2 (svPLA2) Activity from the Venom of Javan Spitting Cobra, Naja sputatrix, based on Body Scales Color and Storage Condition

Nia Kurniawan, Dea Jolie Chrestella, Dinda Sherlyndra Hapsari, Fatchiyah

2019

Abstract

Naja sputatrix (Javan spitting cobra) is one of medically important snake species in Indonesia which have various dorsal scales color. This research purposes to examine the differences of venom genera l profile and its phospholipase A2 (svPLA2) activity from some N. sputatrix with different dorsal scales colors, and to examine the activity of N. sputatrix svPLA2 in different storage conditions.A total of 6 N. sputatrix from East Java with various dorsal scales color were milked. Venom storage was performed at -80, 4 and 37°C in a maximum period of 14 days. Venom profile and phospholipase A2 activity were examined through 15% SDS-PAGE and acidimetric method using egg yolk substrate respectively. Statistical analyses were performed to evaluate svPLA2 activity in every dorsal color and storage condition. Few protein bands range in 16 – 22 kDa are only found in the venom of the certain dorsal color snake. Protein bands at 37°C were found to have the lightest intensity among other groups. The svPLA2 activity of brown dorsal N. sputatrix is found as the highest activity. An interaction between the storage temperature factor and period factor has effects on post-storage svPLA2 activity. Storage in 37°C effects on svPLA2 activity declining compared to the control group and other experimental groups.

Paper Citation

in Harvard Style

Kurniawan N., Chrestella D., Hapsari D. and F. (2019). The Differences of Electrophoretic Profile and Snake Venom Phospholipase A2 (svPLA2) Activity from the Venom of Javan Spitting Cobra, Naja sputatrix, based on Body Scales Color and Storage Condition.In Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering - Volume 1: ICAMBBE, ISBN 978-989-758-483-1, pages 19-28. DOI: 10.5220/0009587600190028

in Bibtex Style

@conference{icambbe19,
author={Nia Kurniawan and Dea Jolie Chrestella and Dinda Sherlyndra Hapsari and Fatchiyah },
title={The Differences of Electrophoretic Profile and Snake Venom Phospholipase A2 (svPLA2) Activity from the Venom of Javan Spitting Cobra, Naja sputatrix, based on Body Scales Color and Storage Condition},
booktitle={Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering - Volume 1: ICAMBBE,},
year={2019},
pages={19-28},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0009587600190028},
isbn={978-989-758-483-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering - Volume 1: ICAMBBE,
TI - The Differences of Electrophoretic Profile and Snake Venom Phospholipase A2 (svPLA2) Activity from the Venom of Javan Spitting Cobra, Naja sputatrix, based on Body Scales Color and Storage Condition
SN - 978-989-758-483-1
AU - Kurniawan N.
AU - Chrestella D.
AU - Hapsari D.
AU - F.
PY - 2019
SP - 19
EP - 28
DO - 10.5220/0009587600190028