In-vivo Test of Chlorella Protein Fragments as Nucleotide Vaccine Candidates in Grouper Viral Nervous Necrosis (VNN) Infection against Haematological Response

Uun Yanuhar, Muhammad Musa, Diana Arfiati, Nico Rahman Caesar, Nur Sakinah Junirahma

2019

Abstract

Grouper (Cromileptes altivelis) is a species of fish with important economic values both in the national and international markets. The disease that has been reported by researchers is Viral Nervous Necrosis (VNN) which can cause mass death in groupers, especially in larval and juvenile stadia. Based on the problems, a research is needed on haematological analysis of groupers (Cromileptes altivelis) infected with Viral Nervous Necrosis by in-vivo testing using protein fragments of C. vulgaris. This research employed an experiment method using 5 treatments, namely (A) healthy fish, (B) VNN-infected fish, (C) VNN-infected fish with administration of C.vulgaris crude extracts of 17 µg mL-1, (D) VNN-infected fish with administration of C.vulgaris crude extract of 33 µg mL-1, and (E) VNN-infected fish with administration of C.vulgaris crude extract of 50 µg mL-1. Observations of haematological parameters included erythrocytes, leukocytes, haemoglobin, and haematocrit. The observation results showed an erythrocyte value of 97 x 104 cells/mm3 in treatment (C), 107 x 104 cells/mm3 in treatment (D), and 94 x 104 cells/mm3 in treatment (E). The observation results of leukocyte values were 150,000 cells/mm3 in treatment (C), 133,300 cells/mm3 in treatment (D), and 139,000 cells/mm3 in treatment (E). Furthermore, the observation results of haemoglobin showed a value of 5 gr/100 ml in treatment (C), 6 gr/100 ml in treatment (D), and 5 gr/100 ml in treatment (E). As for the haematocrit parameter, the results obtained from the observation were 18% in treatment (C), 22% in treatment (D), and 15% in treatment (E). Based on this research, the haematological status of VNN-infected groupers was not good. However, the results of the in-vivo testing conducted showed that administration of C. vulgaris extract gave a positive result on improving the haematological status of groupers (C. altivelis) infected with VNN with the optimal dose of 33 µg mL-1.

Paper Citation

in Harvard Style

Yanuhar U., Musa M., Arfiati D., Caesar N. and Junirahma N. (2019). In-vivo Test of Chlorella Protein Fragments as Nucleotide Vaccine Candidates in Grouper Viral Nervous Necrosis (VNN) Infection against Haematological Response.In Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering - Volume 1: ICAMBBE, ISBN 978-989-758-483-1, pages 79-83. DOI: 10.5220/0009588100790083

in Bibtex Style

@conference{icambbe19,
author={Uun Yanuhar and Muhammad Musa and Diana Arfiati and Nico Rahman Caesar and Nur Sakinah Junirahma},
title={In-vivo Test of Chlorella Protein Fragments as Nucleotide Vaccine Candidates in Grouper Viral Nervous Necrosis (VNN) Infection against Haematological Response},
booktitle={Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering - Volume 1: ICAMBBE,},
year={2019},
pages={79-83},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0009588100790083},
isbn={978-989-758-483-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical Engineering - Volume 1: ICAMBBE,
TI - In-vivo Test of Chlorella Protein Fragments as Nucleotide Vaccine Candidates in Grouper Viral Nervous Necrosis (VNN) Infection against Haematological Response
SN - 978-989-758-483-1
AU - Yanuhar U.
AU - Musa M.
AU - Arfiati D.
AU - Caesar N.
AU - Junirahma N.
PY - 2019
SP - 79
EP - 83
DO - 10.5220/0009588100790083