Puguntano Extract Supplementation Enhances Insulin Secretion and Decreases Insulin Resistance in High Fat Diet/Streptozotocin- Induced Type 2 Diabetic Rats

Santi Syafril, Dharma Lindarto

2019

Abstract

BACKGROUND: Secondary metabolites of puguntano (Curangafel-terrae Merr.) which contains flavonoids, saponins, tannins, and steroids/ terpenoids, increased insulin secretion and decreased insulin resistance in type 2 diabetes mellitus (T2DM). AIM: This study investigated the effect of puguntano leaf extract to insulin secretion and insulin resistance in high-fat diet/streptozotocin-induced type 2 diabetic rats. METHODS: A combination of high-fat diet-feeding (HFD) and multiple low dose intraperitoneal injections of streptozotocin(STZ) was used to induced T2DM in 48 male Wistar rats, which were then randomly divided into control and treatment groups (n = 24 per group). Puguntano leaf extract was administered to the treatment group once daily (200 mg/kg.bw) for 10 days. Insulin secretion (HOMA-) and insulin resistance (HOMA-IR) were measured from fasting glucose and fasting serum insulin concentration by homeostasis model assessment (HOMA) in control group after becoming T2DM and in the treatment group after 10 days of puguntano treatment. Data were analyzed using the Wilcoxon test. RESULTS: HOMA- was significantly higher in the treatment group than in the control group, while HOMA-IR was significantly lower in the treatment group than in the control group (p<0.05). CONCLUSION: This study demonstrated that puguntano leaf extract treatment may significantly enhance insulin secretion and decreased insulin resistance in T2DM rats.

Paper Citation

in Harvard Style

Syafril S. and Lindarto D. (2019). Puguntano Extract Supplementation Enhances Insulin Secretion and Decreases Insulin Resistance in High Fat Diet/Streptozotocin- Induced Type 2 Diabetic Rats.In Proceedings of the 2nd International Conference on Tropical Medicine and Infectious Disease - Volume 1: ICTROMI, ISBN 978-989-758-469-5, pages 137-141. DOI: 10.5220/0009862001370141

in Bibtex Style

@conference{ictromi19,
author={Santi Syafril and Dharma Lindarto},
title={Puguntano Extract Supplementation Enhances Insulin Secretion and Decreases Insulin Resistance in High Fat Diet/Streptozotocin- Induced Type 2 Diabetic Rats},
booktitle={Proceedings of the 2nd International Conference on Tropical Medicine and Infectious Disease - Volume 1: ICTROMI,},
year={2019},
pages={137-141},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0009862001370141},
isbn={978-989-758-469-5},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd International Conference on Tropical Medicine and Infectious Disease - Volume 1: ICTROMI,
TI - Puguntano Extract Supplementation Enhances Insulin Secretion and Decreases Insulin Resistance in High Fat Diet/Streptozotocin- Induced Type 2 Diabetic Rats
SN - 978-989-758-469-5
AU - Syafril S.
AU - Lindarto D.
PY - 2019
SP - 137
EP - 141
DO - 10.5220/0009862001370141