The Effectiveness of Immunostimulant from Ethanol Extract of Parasite Coffee Leaves in Male Rat with Slow Type Hypersensitivity Method

Rahmad Gurusinga, Tati Murni Karokaro, Iskandar Markus Sembiring, Sri Wulan, Novita Br Ginting Munthe, Romauli Anna Teresia Marbun, Suci Wulandari

2019

Abstract

This research aimed to determine the effectiveness of immunostimulant from ethanol extract of parasite coffee leaves with four dose variants. This research used 18 male white rats with 200 grams of weight divided into 6 treatment groups. The rats were induced with Escherichia coli for 7 consecutive days intra- peritoneum, then on the 7th day gave a mark on the rat's feet that had been measured (V0). Ethanol extract of parasite coffee leaves with 50mg / kg weight, 100mg / kg weight, 200mg / kg weight, 400mg / kg weight, 0.5% CMC Na suspension, and 25 mg / kg weight STIMUNO suspension as positive control were given orally on 8th day, after 24 hours of administration the volume of the rat's feet was measured again, then all data from each group was processed by using the ANOVA test. The results showed that the ethanol extract of the parasite coffee leaves proved to be effective as an immunostimulant. This is attested by reduction in the volume of swelling on rat's feet which tested by the slow type hypersensitivity. It was said to be effective because it had an approaching value positive control that is 25 mg stimuno starting from 200 mgkg / weight dose to 400 mgkg / weight. With an average reduction in the volume of swelling 1.4 mm for dosage of 400 mgkg / weight and 1.2 mm for dosage of 200 mgkg / weight.

Paper Citation

in Harvard Style

Gurusinga R., Karokaro T., Sembiring I., Wulan S., Munthe N., Marbun R. and Wulandari S. (2019). The Effectiveness of Immunostimulant from Ethanol Extract of Parasite Coffee Leaves in Male Rat with Slow Type Hypersensitivity Method.In Proceedings of the International Conference on Health Informatics and Medical Application Technology - Volume 1: ICHIMAT, ISBN 978-989-758-460-2, pages 499-506. DOI: 10.5220/0009973504990506

in Bibtex Style

@conference{ichimat19,
author={Rahmad Gurusinga and Tati Murni Karokaro and Iskandar Markus Sembiring and Sri Wulan and Novita Br Ginting Munthe and Romauli Anna Teresia Marbun and Suci Wulandari},
title={The Effectiveness of Immunostimulant from Ethanol Extract of Parasite Coffee Leaves in Male Rat with Slow Type Hypersensitivity Method},
booktitle={Proceedings of the International Conference on Health Informatics and Medical Application Technology - Volume 1: ICHIMAT,},
year={2019},
pages={499-506},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0009973504990506},
isbn={978-989-758-460-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the International Conference on Health Informatics and Medical Application Technology - Volume 1: ICHIMAT,
TI - The Effectiveness of Immunostimulant from Ethanol Extract of Parasite Coffee Leaves in Male Rat with Slow Type Hypersensitivity Method
SN - 978-989-758-460-2
AU - Gurusinga R.
AU - Karokaro T.
AU - Sembiring I.
AU - Wulan S.
AU - Munthe N.
AU - Marbun R.
AU - Wulandari S.
PY - 2019
SP - 499
EP - 506
DO - 10.5220/0009973504990506