Phytochemical Screening of Phenolic Levels from Extracted Bitter Mustard Leaves (Brassica juncen L. Czern.) using UV-Visible Spectrophotometer

Fahma Shufyani, David Ginting, Jhon Patar Sinurat, Tika Afriani, Muhammad Mabrur, Ritmah Syah Putri

2019

Abstract

To determine the total phenolic content of the ethyl acetate extract of bitter mustard leaf. To determine the total phenolic levels, the Folin – Ciocalteu method with gallic acid comparison compounds using ethyl acetate solvents using UV-Visible spectrophotometer is expected to provide information and scientific evidence to develop new medicines from this plant. Antioxidants are inhibitors of oxidation reactions due to free radicals that can cause damage to unsaturated fatty acids, cell wall membranes, blood vessels, DNA bases, and lipid tissue causing disease. Phytochemical screening was using FeCl3 5% to identify phenolic compound. Total phenolic is gotten by many process such maceration and partition. Analysis of total phenolic was doing by TLC analysis. Total phenolic was analysed on plate of thin layer chromatography with used chloroform and methanol with 70:30 comparison. Antioxidant test was measured by UV-Visible spectrophotometer at 516 nm wavelength. Bitter mustard leaf extract contains phenolic compounds based on phytochemical screening results. Weight total phenolic content o 39.7252 ± 0.7326 mg GAE/g extract, the total phenol content determined according to the Folin-Ciocalteu medthod is not an absolute level, but principally based on the reduction capacity of the material being tested against an equivalent reduction of gallic acid. Calibration curve measurement with a concentrations of 200, 225, 250, 275 and 300 μg/ml. All solutions were measured at a wavelength of 739.50 nm. Phytochemical screening results show that the extract of bitter mustard leaf (Brassica juncea L.), contains chemicals, flavonoids, tannins, saponins, glycosides, steroids / triterpenoids, anthraquinone and polyphenols. The acetyl extract of bitter mustard leaf has a total phenolic activity value (39.7252 ± 0.7326) mg GAE / g extract. Penol compounds are susceptible to oxidation at higher temperature in extractions that are to long can provide opportunities for phenol compounds to oxidize more, but measured total phenol levels can be lower.

Paper Citation

in Harvard Style

Shufyani F., Ginting D., Sinurat J., Afriani T., Mabrur M. and Putri R. (2019). Phytochemical Screening of Phenolic Levels from Extracted Bitter Mustard Leaves (Brassica juncen L. Czern.) using UV-Visible Spectrophotometer.In Proceedings of the International Conference on Health Informatics and Medical Application Technology - Volume 1: ICHIMAT, ISBN 978-989-758-460-2, pages 507-514. DOI: 10.5220/0009973605070514

in Bibtex Style

@conference{ichimat19,
author={Fahma Shufyani and David Ginting and Jhon Patar Sinurat and Tika Afriani and Muhammad Mabrur and Ritmah Syah Putri},
title={Phytochemical Screening of Phenolic Levels from Extracted Bitter Mustard Leaves (Brassica juncen L. Czern.) using UV-Visible Spectrophotometer},
booktitle={Proceedings of the International Conference on Health Informatics and Medical Application Technology - Volume 1: ICHIMAT,},
year={2019},
pages={507-514},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0009973605070514},
isbn={978-989-758-460-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the International Conference on Health Informatics and Medical Application Technology - Volume 1: ICHIMAT,
TI - Phytochemical Screening of Phenolic Levels from Extracted Bitter Mustard Leaves (Brassica juncen L. Czern.) using UV-Visible Spectrophotometer
SN - 978-989-758-460-2
AU - Shufyani F.
AU - Ginting D.
AU - Sinurat J.
AU - Afriani T.
AU - Mabrur M.
AU - Putri R.
PY - 2019
SP - 507
EP - 514
DO - 10.5220/0009973605070514