Antioxidant of Total Phenolic from Saputangan Leaves (Maniltoa grandiflora (A. Gray) Scheff)

Jhon Patar Sinurat, Reh Malem Br Karo, Saadah Siregar, Romauli Anna Teresia Marbun, Fahma Shufyani

2019

Abstract

Phytochemical screening test used 5% FeCl3 reagent showed extract become to black extract that saputangan leaves contained phenolic compound. Saputangan leaves powder was macerated with methanol and got macerate as 100.84 g. Macerate was dissolved with aquadest to remove lipid in saputangan leaves. First partition was used ethyl acetate to get solid extract as 37.03 g. Second Partition was used n-hexane to get solid extract as 18.25 g. Total phenolic was analysed on plate of thin layer chromatography with used chloroform and methanol with 70:30 comparison. Total phenolic has 3 spots that have an Rf of 0.44 ; 0.29 and 0.22. Total phenolic absorbance measured at 516 nm wavelength using a UV-Visible spectrophotometer. Total phenolic solutions were soluted in concentrations of 10, 25 and 50 ppm using methanol p.a as solvent. The absorbance obtained at all three concentrations was 0.202; 0.195 and 0.175. Based on the linear regression equation Y = 1,191 X + 31.87, the IC50 value for the total phenolic was 15.22 ppm. IC50 value of 15.22 ppm can be categorized as a total phenolic compound as a strong antioxidant.

Paper Citation

in Harvard Style

Sinurat J., Br Karo R., Siregar S., Marbun R. and Shufyani F. (2019). Antioxidant of Total Phenolic from Saputangan Leaves (Maniltoa grandiflora (A. Gray) Scheff).In Proceedings of the International Conference on Health Informatics and Medical Application Technology - Volume 1: ICHIMAT, ISBN 978-989-758-460-2, pages 536-542. DOI: 10.5220/0009974405360542

in Bibtex Style

@conference{ichimat19,
author={Jhon Patar Sinurat and Reh Malem Br Karo and Saadah Siregar and Romauli Anna Teresia Marbun and Fahma Shufyani},
title={Antioxidant of Total Phenolic from Saputangan Leaves (Maniltoa grandiflora (A. Gray) Scheff)},
booktitle={Proceedings of the International Conference on Health Informatics and Medical Application Technology - Volume 1: ICHIMAT,},
year={2019},
pages={536-542},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0009974405360542},
isbn={978-989-758-460-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the International Conference on Health Informatics and Medical Application Technology - Volume 1: ICHIMAT,
TI - Antioxidant of Total Phenolic from Saputangan Leaves (Maniltoa grandiflora (A. Gray) Scheff)
SN - 978-989-758-460-2
AU - Sinurat J.
AU - Br Karo R.
AU - Siregar S.
AU - Marbun R.
AU - Shufyani F.
PY - 2019
SP - 536
EP - 542
DO - 10.5220/0009974405360542