Difference in Characteristic of Beetroot (Beta Vulgaris L.) Biomass through Microfiltration Technique to Prevent Natural Infection

Agustine Susilowati, Aspiyanto, Hani Mulyani, Puspa Lotulung, Yati Maryati

2019

Abstract

Fermentation of beetroot (Beta vulgaris L.) by using Kombucha culture produces biomass as a source of polyphenol, particularly betacyanins having potential use as prevention of natural infection. This experiment activity aims to find out separation optimization of beetroot biomass through microfiltration (MF) technique and characteristic of retentate and permeate as a result of MF on composition, monomer domination of polyphenol and betacyanin compounds, particle size distribution, and ability of anti-bacteria to prevent natural infection on Staphylococcus aureus Ina CC-B4 and Escherichia coli Ina CC-B5. Separation was conducted at room temperature, strirrer rotation speed (SRS) 200, 300 and 400 rpm, and fixed transmembrane pressure (TMP) 40 psia. Result of experiment work showed that based on betacyanin, the best treatment is achieved at SRS 300 rpm yielding retentate and permeate with composition of betacyanin of 0.31 and 0.16 µg/mL, total polyphenol of 0.55 and 0.37%, total acids 1.00 and 0.72%, reducing sugars 16.33 and 28.97 mg/mL, total solids 10.05 and 9.38%, dissolved protein 18.50 and 24.35 mg/mL, particle size 3002.4 and 1962.0 nm, particle index 0.468 and 0.370, respectively. Identificaion on betacyanin and gallic acid monomers as total polyphenol at retentate is dominated by monomer with molecular weight (MW) 551.13, 551.53, and 171.02, 171.24 and 171.76 Dalton (Da.), meanwhile permeate is dominated by monomer with MW 551.16 and 171.23, 171.72 Da. and relative intensities 100%, respectively. Ability to inhibit the growth of bacteria of Staphylococcus aureus Ina CC-B4 and Escherichia coli Ina CC-B5 is achieved by retentate with zone area of inhibiting 11 and 10 mm, respectively. In this optimum condition, MF membrane technique was able to retain betacyanin and total polyphenol as anti-bacteria compounds in retentate with 416% (4.16-folds) and 83.33% and pass them in permeate 166% (1.67-folds) and 23.33% compared to prior to process (feed).

Paper Citation

in Harvard Style

Susilowati A., Aspiyanto., Mulyani H., Lotulung P. and Maryati Y. (2019). Difference in Characteristic of Beetroot (Beta Vulgaris L.) Biomass through Microfiltration Technique to Prevent Natural Infection. In Proceedings of the 16th ASEAN Food Conference - Volume 1: 16th AFC, ISBN 978-989-758-467-1, pages 301-311. DOI: 10.5220/0009981000002964

in Bibtex Style

@conference{16th afc19,
author={Agustine Susilowati and Aspiyanto and Hani Mulyani and Puspa Lotulung and Yati Maryati},
title={Difference in Characteristic of Beetroot (Beta Vulgaris L.) Biomass through Microfiltration Technique to Prevent Natural Infection},
booktitle={Proceedings of the 16th ASEAN Food Conference - Volume 1: 16th AFC,},
year={2019},
pages={301-311},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0009981000002964},
isbn={978-989-758-467-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 16th ASEAN Food Conference - Volume 1: 16th AFC,
TI - Difference in Characteristic of Beetroot (Beta Vulgaris L.) Biomass through Microfiltration Technique to Prevent Natural Infection
SN - 978-989-758-467-1
AU - Susilowati A.
AU - Aspiyanto.
AU - Mulyani H.
AU - Lotulung P.
AU - Maryati Y.
PY - 2019
SP - 301
EP - 311
DO - 10.5220/0009981000002964