Enhancing Anti-pathogenic Bacteria Activity of Lactobacillus Plantarum AKK-30 Cultured on the Medium Containing Fructose-Oligosaccharides

Nisa Grendpina, Dyah Fitri, Hardi Julendra, Ahmad Sofyan, Ema Damayanti

2019

Abstract

The purpose of this study was to evaluate the concentration of Fructose-Oligosaccharides (FOS) in correlation with incubation time for growing - Lactobacillus plantarum AKK-30, and to assess metabolites of L. plantarum AKK-30 as antimicrobial substances against to pathogenic bacteria. L. plantarum AKK-30 was isolated from the small intestine of native chicken. L. plantarum AKK-30 was grown on MRSB medium containing FOS (0%, 0.5%, 1%, and 1.5%), and incubated at different times (6, 12, 18, and 24 hours) at 37°C. Antimicrobial activity of L. plantarum AKK-30 metabolites was tested on four species of pathogenic bacteria consisted of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Salmonella pullorum. The results showed that the concentration of 1% FOS and 24-hours incubation were most effective in increasing L. plantarum AKK-30 growth (2.11 x 108 CFU/ml). Antimicrobial activity extract of L. plantarum AKK-30 metabolites was able to inhibit the growth of E. coli, P. aeruginosa, S. aureus, and S. pullorum. The highest inhibition of bacteria was observed on S. aureus which was 10.8 mm, followed by E. coli at 9.9 mm, S. pullorum at 9.083 mm, and P. aeruginosa 8.783 mm.

Paper Citation

in Harvard Style

Grendpina N., Fitri D., Julendra H., Sofyan A. and Damayanti E. (2019). Enhancing Anti-pathogenic Bacteria Activity of Lactobacillus Plantarum AKK-30 Cultured on the Medium Containing Fructose-Oligosaccharides. In Proceedings of the 16th ASEAN Food Conference - Volume 1: 16th AFC, ISBN 978-989-758-467-1, pages 205-209. DOI: 10.5220/0009991800002964

in Bibtex Style

@conference{16th afc19,
author={Nisa Grendpina and Dyah Fitri and Hardi Julendra and Ahmad Sofyan and Ema Damayanti},
title={Enhancing Anti-pathogenic Bacteria Activity of Lactobacillus Plantarum AKK-30 Cultured on the Medium Containing Fructose-Oligosaccharides},
booktitle={Proceedings of the 16th ASEAN Food Conference - Volume 1: 16th AFC,},
year={2019},
pages={205-209},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0009991800002964},
isbn={978-989-758-467-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 16th ASEAN Food Conference - Volume 1: 16th AFC,
TI - Enhancing Anti-pathogenic Bacteria Activity of Lactobacillus Plantarum AKK-30 Cultured on the Medium Containing Fructose-Oligosaccharides
SN - 978-989-758-467-1
AU - Grendpina N.
AU - Fitri D.
AU - Julendra H.
AU - Sofyan A.
AU - Damayanti E.
PY - 2019
SP - 205
EP - 209
DO - 10.5220/0009991800002964