Chlorogenic Acid Ameliorates Vascular Remodeling and Perivascular Fibrosis in Kidney Fibrosis Model in Mice

Gabriella Bamba Ratih Lintin, Nur Arfian, Dwi Cahyani Ratna Sari, Gina Andyka Hutasoit, Mohammad Salman, Muhammad Mansyur Romi

2020

Abstract

Kidney Fibrosis is the common pathway final of Chronic Kidney Disease, which is characterized by vascular remodeling and perivascular fibrosis. The Unilateral Ureteral Obstruction (UUO) model is used to cause kidney fibrosis. Chlorogenic Acid (CGA) is an antioxidant as a renoprotective agent. However, fibrosis perivascular and vascular remodeling has not analyzed yet. Study has objective to examine the effect of CGA in vascular aspects, specifically vascular remodeling and perivascular fibrosis in kidney fibrosis. Material and Methods: This research was a true experimental study. Unilateral Ureteral Obstruction (UUO) was performed in swiss webster background mice (n=25, 2-3 months old, 20-30 g weight) to induce kidney fibrosis. The mice were divided into five groups, SO (Sham Operation/control), U7 (UUO day-7), U14 (UUO day-14), UC7 (UUO+CGA day-7), and UC14 (UUO+CGA day-14). CGA 14 mg/kg body weight/day was induced intraperitoneally. Vascular remodeling based on lumen area, mean wall thickness, and wall/lumen area ratio (WLAR) and perivascular fibrosis of intrarenal arteries were quantified using Sirius Red staining. Study found that UUO groups (U7 and U14) had significantly higher vascular remodeling, as shown by lower lumen area, higher mean wall thickness and higher WLAR, and perivascular fibrosis, as shown by higher area compared to SO group (p<0.05). On the other hand, CGA groups (UC7 and UC14) revealed lower vascular remodeling, as shown by higher lumen area, lower WLAR, and perivascular fibrosis, as shown by lower area significantly compared to UUO group (p<0.05). The mean wall thickness was lower, but the data was not significantly different. Study conclude that CGA ameliorates kidney fibrosis through vascular remodeling and perivascular fibrosis.

Paper Citation

in Harvard Style

Lintin G., Arfian N., Ratna Sari D., Hutasoit G., Salman M. and Romi M. (2020). Chlorogenic Acid Ameliorates Vascular Remodeling and Perivascular Fibrosis in Kidney Fibrosis Model in Mice.In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 15-20. DOI: 10.5220/0010487000150020

in Bibtex Style

@conference{jimc20,
author={Gabriella Bamba Ratih Lintin and Nur Arfian and Dwi Cahyani Ratna Sari and Gina Andyka Hutasoit and Mohammad Salman and Muhammad Mansyur Romi},
title={Chlorogenic Acid Ameliorates Vascular Remodeling and Perivascular Fibrosis in Kidney Fibrosis Model in Mice},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={15-20},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010487000150020},
isbn={978-989-758-499-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - Chlorogenic Acid Ameliorates Vascular Remodeling and Perivascular Fibrosis in Kidney Fibrosis Model in Mice
SN - 978-989-758-499-2
AU - Lintin G.
AU - Arfian N.
AU - Ratna Sari D.
AU - Hutasoit G.
AU - Salman M.
AU - Romi M.
PY - 2020
SP - 15
EP - 20
DO - 10.5220/0010487000150020