Immunohistochemistry of KRAS Protein in Colorectal Cancer

Dody Novrial, Kamal Agung Wijayana, Hanif Kun Cahyani

2020

Abstract

PCR assays are used for the detection of KRAS mutations in colorectal cancer (CRC). However, there are some disadvantages in the clinical application such as the high-cost value and specific codon properties of this method. Our study aimed to evaluate the effectivity of immunohistochemistry (IHC) in assessing ras gene aberration in CRC before PCR testing. Paraffin-embedded tissue samples from 45 CRC patients were stained immunohistochemically using anti-KRAS protein polyclonal antibody. KRAS protein expression was assessed and correlated with clinicopathological features. A chi-square test was performed to evaluate the results statistically. KRAS protein positivity was observed in 31.1% of cases. The positivity was correlated with female predominance (p=0.03). No significant correlation between KRAS protein expression with age, tumor topography, lymph node metastases, TNM staging, and tumor differentiation. In conclusion, IHC was regarded as cost- effective, non-specific for codons, and may complement PCR in the detection of KRAS gene mutation.

Paper Citation

in Harvard Style

Novrial D., Wijayana K. and Cahyani H. (2020). Immunohistochemistry of KRAS Protein in Colorectal Cancer.In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 47-51. DOI: 10.5220/0010487500470051

in Bibtex Style

@conference{jimc20,
author={Dody Novrial and Kamal Agung Wijayana and Hanif Kun Cahyani},
title={Immunohistochemistry of KRAS Protein in Colorectal Cancer},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={47-51},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010487500470051},
isbn={978-989-758-499-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - Immunohistochemistry of KRAS Protein in Colorectal Cancer
SN - 978-989-758-499-2
AU - Novrial D.
AU - Wijayana K.
AU - Cahyani H.
PY - 2020
SP - 47
EP - 51
DO - 10.5220/0010487500470051