The Effects of Aqueous Extract of Jamaican Cherry (Muntingia calabura) on D-galactose-induced Liver Damage in BALB/c Mice (Mus musculus)

Cempaka Jaga Pramudita, Dwi Nur Ahsani, Ika Fidianingsih, Evy Sulistyoningrum

2020

Abstract

D-Galactose induces oxidative stress that will damage the hepatocytes. The aqueous extract of Muntingia calabura leaves (AQMC) is known to have a potential antioxidant to prevent hepatocyte damage caused by oxidative stress. This study aimed to determine the effects of AQMC leaf extract on liver damage induced by D-Galactose in BALB/c Mus musculus. This was experimental research with a completely-randomized design involving 20 hepatic samples (paraffin blocks and HE staining) from 5 groups. The groups included K1 (healthy), K2 (D-galactose-induced), K3 (D-galactose + AQMC 35 mg/kgBW), K4 (D-galactose + AQMC 75mg/kgBW), and K5 (D-galactose + vitamin C 28mg/KgBW). D-galactose was administered for 6 weeks prior to the therapy (vitamin C or AQMC given for 4 weeks). Liver damage was observed in all fields of view and described comprehensively. The degree of hepatocyte damage was calculated using the Manja Roenigk scoring and analyzed using One-way ANOVA with post-hoc Tukey’s HSD test (CI = 95%, α = 0.05). AQMC leaf extract could reduce D-galactose-induced liver damage in BALB/c Mus musculus. The hepatocyte damage in the groups given AQMC therapy was less than that in the D-galactose negative control group (K1 = 73.5 + 2.39, K2 = 92.00 + 5.24, K3 = 69.00 + 2.79, K4 = 76.25 + 4.42, K5 = 77.25 + 6.48; p = 0.029). AQMC at a dose of 35mg/KgBW showed more effective therapeutic potential against D-galactose (K3 = 0.003, K4 = 0.027; post-hoc toward K2). AQMC administration could reduce liver and hepatocyte damage of BALB/c Mus musculus induced by D-Galactose at a potential dose of 35 mg.

Paper Citation

in Harvard Style

Pramudita C., Ahsani D., Fidianingsih I. and Sulistyoningrum E. (2020). The Effects of Aqueous Extract of Jamaican Cherry (Muntingia calabura) on D-galactose-induced Liver Damage in BALB/c Mice (Mus musculus).In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 202-207. DOI: 10.5220/0010490102020207

in Bibtex Style

@conference{jimc20,
author={Cempaka Jaga Pramudita and Dwi Nur Ahsani and Ika Fidianingsih and Evy Sulistyoningrum},
title={The Effects of Aqueous Extract of Jamaican Cherry (Muntingia calabura) on D-galactose-induced Liver Damage in BALB/c Mice (Mus musculus)},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={202-207},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010490102020207},
isbn={978-989-758-499-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - The Effects of Aqueous Extract of Jamaican Cherry (Muntingia calabura) on D-galactose-induced Liver Damage in BALB/c Mice (Mus musculus)
SN - 978-989-758-499-2
AU - Pramudita C.
AU - Ahsani D.
AU - Fidianingsih I.
AU - Sulistyoningrum E.
PY - 2020
SP - 202
EP - 207
DO - 10.5220/0010490102020207