Reversible Imiquimod Effects on Skin Tissue of Psoriasis Mice Model: An Experimental Study

Thianti Sylviningrum, Afifah, Dody Novrial, Brian Wasita, Bambang Purwanto, Harijono K. Sentono

2020

Abstract

Psoriasis is an incurable inflammatory disease with erythematous, scaly, and thick skin. Imiquimod (IMQ)-induced psoriasis mice have been widely used since it may resemble psoriasis human. Imiquimod effects on development, peak, and resolution of mice skin are crucial. Thus, this study aims at evaluating IMQ effects on psoriasis mice model skin tissue changes. Twenty-seven female Balb/c mice 8-11 weeks, 20-25 grams body weight (BW) were randomize equally into control (A) and experimental groups (B,C). Over 25 grams BW mice after acclimatization were excluded. The mice had shaved their back then topically applied emollient (A) and IMQ (B,C) for 7 consecutive days. On day 8 (A,B) and 15 (C), mice were terminated and back skin harvested for histopathological examination. Psoriasis Area Severity Index (PASI) and Baker’s scores were used to measure macroscopic and microscopic skin changes. All groups score differences were assessed using Kruskal Wallis then Mann Whitney tests. Skin changes gradually appeared from day 2 to 7 of IMQ applications and faded after 2 days IMQ discontinuation. On day 7, all mice showed means of PASI scores 0(A);9,00±2,69(B);8,11±1,62(C) with significant differences between experimental to control groups (p = 0.00). Means of PASI scores from mice group C on day 15 showed similar to group A (p = 0.10). Means of Baker’s scores from all groups were 1,16±0,25(A); 3,33±1,25(B);1,39±0,22(C) and group B mice showed different significant scores from others (p = 0.00). As conclusion, the IMQ showed a reversible effect on skin tissue changes in psoriasis mice model.The abstract should summarize the contents of the paper and should contain at least 70 and at most 200 words. It should be set in 9-point font size, justified and should have a hanging indent of 2-centimenter. There should be a space before of 12-point and after of 30-point.

Paper Citation

in Harvard Style

Sylviningrum T., Afifah., Novrial D., Wasita B., Purwanto B. and Sentono H. (2020). Reversible Imiquimod Effects on Skin Tissue of Psoriasis Mice Model: An Experimental Study.In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 260-266. DOI: 10.5220/0010491002600266

in Bibtex Style

@conference{jimc20,
author={Thianti Sylviningrum and Afifah and Dody Novrial and Brian Wasita and Bambang Purwanto and Harijono K. Sentono},
title={Reversible Imiquimod Effects on Skin Tissue of Psoriasis Mice Model: An Experimental Study},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={260-266},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010491002600266},
isbn={978-989-758-499-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - Reversible Imiquimod Effects on Skin Tissue of Psoriasis Mice Model: An Experimental Study
SN - 978-989-758-499-2
AU - Sylviningrum T.
AU - Afifah.
AU - Novrial D.
AU - Wasita B.
AU - Purwanto B.
AU - Sentono H.
PY - 2020
SP - 260
EP - 266
DO - 10.5220/0010491002600266