Secretion of IFN-γ and IL-17 after Stimulation of ESAT-6-CFP10 (EC610) Fusion Antigen from PBMC in Groups Active TB and Latent TB

Nika Andriani, Nova Kurniati, Muhammad Irsan Saleh, Eddy Mart Salim, Zen Hafy, Jusak Nugraha, Kemas Ya’kub Rahadiyanto, Francisca Srioetami Tanoerahardjo

2020

Abstract

Tuberculosis is an infectious disease that is transmitted by the bacteria Mycobacterium tuberculosis. The immune system has an important role in the pathogenesis of TB. The protective response to TB involves the secretion of proinflammatory cytokines, namely Th1 cells that produce IFN-γ and Th 17 which produce IL-17, which plays a very important role in the body's defense system, especially in dealing with intracellular bacterial infections. The EC610 fusion antigen is a specific M.TB antigen which has antigenicity to T cells so that T cells secrete cytokines. The study aimed to determine the immune response of proinflammatory cytokines against Mycobacterium tuberculosis infection by looking at the secretion of IFN-γ and IL-17 levels after stimulation of the ESAT 6-CFP 10 (EC610) fusion antigen in active and latent TB patients. This type of research was a quasi experimental in vitro. The research was conducted at the Palembang Lung Special Hospital. The research subjects were 21 samples of active TB and 28 samples of latent TB. PBMC blood samples were isolated using Ficoll-Paque, induced with ESAT-6 - CFP-10 Fusion Antigen (EC610) for 24 - 72 hours at 37 ° C. IFN-γ and IL-17 were measured by ELISA Reader. Analysis used the Mann Whitney test ρ <0.05. IFN-γ levels and IL-17 levels in active TB were higher than latent TB, but statistically there was no significant difference between IFN-levels (ρ = 0.769) and IL-17 levels with a value of ρ = 0.000, meaning that there was a significant difference between both groups. The cut-off points were IFN-γ (6850 pg / mL) and IL-17 (85 pg / mL) using Receiver Operating Curve (ROC) curve analysis. As a conclusions, IFN levels were not different and IL-17 levels were different. This shows that IL-17 levels play a role in the protective immune response against Mycobacterium tuberculosis during the progression of TB disease.

Paper Citation

in Harvard Style

Andriani N., Kurniati N., Saleh M., Salim E., Hafy Z., Nugraha J., Rahadiyanto K. and Tanoerahardjo F. (2020). Secretion of IFN-γ and IL-17 after Stimulation of ESAT-6-CFP10 (EC610) Fusion Antigen from PBMC in Groups Active TB and Latent TB.In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 289-297. DOI: 10.5220/0010491602890297

in Bibtex Style

@conference{jimc20,
author={Nika Andriani and Nova Kurniati and Muhammad Irsan Saleh and Eddy Mart Salim and Zen Hafy and Jusak Nugraha and Kemas Ya’kub Rahadiyanto and Francisca Srioetami Tanoerahardjo},
title={Secretion of IFN-γ and IL-17 after Stimulation of ESAT-6-CFP10 (EC610) Fusion Antigen from PBMC in Groups Active TB and Latent TB},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={289-297},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010491602890297},
isbn={978-989-758-499-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - Secretion of IFN-γ and IL-17 after Stimulation of ESAT-6-CFP10 (EC610) Fusion Antigen from PBMC in Groups Active TB and Latent TB
SN - 978-989-758-499-2
AU - Andriani N.
AU - Kurniati N.
AU - Saleh M.
AU - Salim E.
AU - Hafy Z.
AU - Nugraha J.
AU - Rahadiyanto K.
AU - Tanoerahardjo F.
PY - 2020
SP - 289
EP - 297
DO - 10.5220/0010491602890297