The Effectiveness of Mesenchymal Stem Cell and Colostrum Bovine Combination in Post Hepatectomy Liver Failure with Liver Fibrosis Animal Model

Dimas Aryo Kusumo, Agung Putra, Albertus Ari Adrianto, Erik Prabowo, Ignatius Riwanto

2020

Abstract

Post hepatectomy liver fibrosis (PHLF) is the most common cause of morbidity and mortality after liver surgery. Recently, mesenchymal stem cells (MSCs) and bovine colostrum (BC) have been studied to exert an anti-fibrotic. However, the effect of MSCs, BC, and their combination of tissue regeneration after PHLF remains unclear. This study aimed to evaluate the effect of MSCs, BC and its combination in regulating transforming growth factor-β (TGF-β) and serum glutamic pyruvic transaminase (SGPT) associated with liver destruction inhibition on PHLF animal models. Eighteen Sprague-Dawley were injected with CCl4 for eight weeks and 50% liver resection (LR). Mice were divided into three groups: group NaCl 0.9 % with parenchymal injection, group MSC with parenchymal injection, and a group of MSCs with parenchymal injection and BC oral. After administration, the level TGF β and SGPT were collected on the 3rd and 7th days. This study showed that the TGF-β levels nit significant decrease under combination treatment compared to the MSCs group until 34,11pg/mL ±4,18 and 34,5 pg/mL ±2.94, respectively, on day 7 th . Besides, SPGT levels of combination treatment also did not significantly decrease compared to the MSCs group until 93,6 U/L ±23,8 and 163,2U/l ±73,62, respectively, on day 3 rd . In conclusion, the combination therapy is no better than single MSCs treatment in regenerating liver tissue on PHLF.

Paper Citation

in Harvard Style

Kusumo D., Putra A., Adrianto A., Prabowo E. and Riwanto I. (2020). The Effectiveness of Mesenchymal Stem Cell and Colostrum Bovine Combination in Post Hepatectomy Liver Failure with Liver Fibrosis Animal Model.In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 305-310. DOI: 10.5220/0010491803050310

in Bibtex Style

@conference{jimc20,
author={Dimas Aryo Kusumo and Agung Putra and Albertus Ari Adrianto and Erik Prabowo and Ignatius Riwanto},
title={The Effectiveness of Mesenchymal Stem Cell and Colostrum Bovine Combination in Post Hepatectomy Liver Failure with Liver Fibrosis Animal Model},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={305-310},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010491803050310},
isbn={978-989-758-499-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - The Effectiveness of Mesenchymal Stem Cell and Colostrum Bovine Combination in Post Hepatectomy Liver Failure with Liver Fibrosis Animal Model
SN - 978-989-758-499-2
AU - Kusumo D.
AU - Putra A.
AU - Adrianto A.
AU - Prabowo E.
AU - Riwanto I.
PY - 2020
SP - 305
EP - 310
DO - 10.5220/0010491803050310