Detection of Rat (Rattus Norvegicus) DNA Fragments using Specific: Species Primer mt-DNA 12S rRNA and Cyt-b with Polymerase Chain Reaction (PCR) Technique

Ersita Aisah, Joni Kusnadi, Joni Kusnadi

2020

Abstract

A pair of species-specific mt-DNA primer 12S rRNA has been designed based on rat (Rattus norvegicus) DNA sequences. In this study, the specificity test of 12S rRNA and Cyt-b were carried out to detect rat DNA fragments. Samples of this research were used non-halal animal meat consisted of rats, lamb, dog, pig and halal animal meat consisted of cow, chicken, and horse. Furthermore, DNA was isolated from animal meat using modification of chloroform isoamyl alcohol method then quantitatively tested for its concentration and purity. Animal meat isolates were amplified using 12S rRNA and Cyt-b primers using PCR techniques. Analysis of PCR results using agarose gel electrophoresis 1.5%. The amplification results showed that 12S rRNA primer produced DNA bands of 228 bp length and Cyt-b primer produced DNA bands of 603 bp length. The amplification results showed that both of 12S rRNA and Cyt-b primers were specific to detect rat DNA fragments. Thus, both of primers are recommended to be further tested for sensitivity and applied to processed meat products such as meatballs, sausages, and corned beef.

Paper Citation

in Harvard Style

Aisah E. and Kusnadi J. (2020). Detection of Rat (Rattus Norvegicus) DNA Fragments using Specific: Species Primer mt-DNA 12S rRNA and Cyt-b with Polymerase Chain Reaction (PCR) Technique. In Proceedings of the 6th Food Ingredient Asia Conference - Volume 1: 6th FiAC, ISBN 978-989-758-540-1, pages 20-24. DOI: 10.5220/0010507100003108

in Bibtex Style

@conference{6th fiac20,
author={Ersita Aisah and Joni Kusnadi},
title={Detection of Rat (Rattus Norvegicus) DNA Fragments using Specific: Species Primer mt-DNA 12S rRNA and Cyt-b with Polymerase Chain Reaction (PCR) Technique},
booktitle={Proceedings of the 6th Food Ingredient Asia Conference - Volume 1: 6th FiAC,},
year={2020},
pages={20-24},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010507100003108},
isbn={978-989-758-540-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 6th Food Ingredient Asia Conference - Volume 1: 6th FiAC,
TI - Detection of Rat (Rattus Norvegicus) DNA Fragments using Specific: Species Primer mt-DNA 12S rRNA and Cyt-b with Polymerase Chain Reaction (PCR) Technique
SN - 978-989-758-540-1
AU - Aisah E.
AU - Kusnadi J.
PY - 2020
SP - 20
EP - 24
DO - 10.5220/0010507100003108