Effects of Electrical Fields on Neuroblastoma (N2A) Cell Differentiation: Preliminary Results

Daniel Martin Fernández, Pablo Pérez García, María E. Martín, Paula Daza, Juan Alfonso Serrano-Viseas, Gloria Huertas, Alberto Yúfera

2021

Abstract

This work describes Electrical Stimulations (ES) assays on stem cells. The neuroblastoma (N2A) cell linage was submitted to several electrical fields to enable and enhance its differentiation toward neurons. Both Direct Current (DC) and Alternated Current (AC) time dependent electric field protocols were applied to N2A cell culture under differentiation conditions, obtaining different responses. Control and electrically excited samples’ number of differentiated cells and neurite lengths were measure after differentiation. Results showed that DC fields have a strong influence on N2A differentiation since the percentage of differentiated cells and the neurites lengths were the highest. In addition, a significant alignment of neurites measured with the applied electrical field has been detected, which demonstrates the high sensitivity of differentiation processes to electrical field polarity.

Paper Citation

in Harvard Style

Martin Fernández D., García P., Martín M., Daza P., Serrano-Viseas J., Huertas G. and Yúfera A. (2021). Effects of Electrical Fields on Neuroblastoma (N2A) Cell Differentiation: Preliminary Results. In Proceedings of the 14th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2021) - Volume 1: BIODEVICES; ISBN 978-989-758-490-9, SciTePress, pages 152-159. DOI: 10.5220/0010320100002865

in Bibtex Style

@conference{biodevices21,
author={Daniel Martin Martin Fernández and Pablo Pérez García and María E. Martín and Paula Daza and Juan Alfonso Serrano-Viseas and Gloria Huertas and Alberto Yúfera},
title={Effects of Electrical Fields on Neuroblastoma (N2A) Cell Differentiation: Preliminary Results},
booktitle={Proceedings of the 14th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2021) - Volume 1: BIODEVICES},
year={2021},
pages={152-159},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010320100002865},
isbn={978-989-758-490-9},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 14th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2021) - Volume 1: BIODEVICES
TI - Effects of Electrical Fields on Neuroblastoma (N2A) Cell Differentiation: Preliminary Results
SN - 978-989-758-490-9
AU - Martin Fernández D.
AU - García P.
AU - Martín M.
AU - Daza P.
AU - Serrano-Viseas J.
AU - Huertas G.
AU - Yúfera A.
PY - 2021
SP - 152
EP - 159
DO - 10.5220/0010320100002865
PB - SciTePress