Anti-inflammatory Activities of Pineapple (Ananas comosus) Core Extract in Lipopolysaccharide-induced RAW264.7 Cell Line

Hanna Kusuma, Hartini Tiono, Philips Onggowidjaja, Selonan Obeng, Wahyu Widowati, Cintani Wahyuni, Cahyaning Wijayanti, Muhamad Maulana, Tri Handayani, Rizal Rizal, Rizal Rizal

2021

Abstract

BACKGROUND: Inflammation is a biological response process by the immune system that may induce acute/chronic inflammatory and leading tissue damage or diseases. Pineapple (Ananas comosus) cores that have been investigated for anti-inflammatory properties and immunomodulator. OBJECTIVE: This research aims to evaluate the anti-inflammatory potency of pineapple core extract (PCE) in lipopolysaccharide-induced macrophage cells (RAW 264.7). METHODS: The viability assay of PCE was determined by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) to ensure the safe and non-toxic concentration in RAW 264.7 cells. The pro-inflammatory induction of cells using 200 μL of lipopolysaccharide (LPS). Levels of PGE-2 and pro-inflammatory cytokines IL-1β and TNF-α levels were measured using enzyme-linked immunosorbent assay. RESULTS: PCE 4 and 20 µg/mL showed high viability (>90\%) with the values 95.03\% and 92.94\%, respectively. PCE 20 µg/mL showed the lower of PGE-2 and TNF-α levels (507.68 pg/mL; 345.90 pg/mL) compared to PCE 4 µg/mL (795.37 pg/mL; 474.19 pg/mL) and positive control (870.48 pg/mL; 581.71 pg/mL). In IL-1β level, PCE 20 µg/mL showed the lower (217.63 pg/mL) compared to PCE 4 pg/mL (350.78 pg/mL) and positive control (433.53 pg/mL).

Paper Citation

in Harvard Style

Kusuma H., Tiono H., Onggowidjaja P., Obeng S., Widowati W., Wahyuni C., Wijayanti C., Maulana M., Handayani T. and Rizal R. (2021). Anti-inflammatory Activities of Pineapple (Ananas comosus) Core Extract in Lipopolysaccharide-induced RAW264.7 Cell Line. In Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - Volume 1: ICE-TES, ISBN 978-989-758-601-9, pages 58-64. DOI: 10.5220/0010744300003113

in Bibtex Style

@conference{ice-tes21,
author={Hanna Kusuma and Hartini Tiono and Philips Onggowidjaja and Selonan Obeng and Wahyu Widowati and Cintani Wahyuni and Cahyaning Wijayanti and Muhamad Maulana and Tri Handayani and Rizal Rizal},
title={Anti-inflammatory Activities of Pineapple (Ananas comosus) Core Extract in Lipopolysaccharide-induced RAW264.7 Cell Line},
booktitle={Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - Volume 1: ICE-TES,},
year={2021},
pages={58-64},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010744300003113},
isbn={978-989-758-601-9},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - Volume 1: ICE-TES,
TI - Anti-inflammatory Activities of Pineapple (Ananas comosus) Core Extract in Lipopolysaccharide-induced RAW264.7 Cell Line
SN - 978-989-758-601-9
AU - Kusuma H.
AU - Tiono H.
AU - Onggowidjaja P.
AU - Obeng S.
AU - Widowati W.
AU - Wahyuni C.
AU - Wijayanti C.
AU - Maulana M.
AU - Handayani T.
AU - Rizal R.
PY - 2021
SP - 58
EP - 64
DO - 10.5220/0010744300003113